1887

Abstract

A psychrotolerant, methylotrophic methanogen, strain YSF-03, was isolated from the saline meromictic Lake Shira in Siberia. Cells of strain YSF-03 were non-motile, irregular cocci and 0.8–1.2 µm in diameter. The methanogenic substrates utilized by strain YSF-03 were methanol and trimethylamine. The temperature range of growth for strain YSF-03 was from 0 to 37 °C. The optimum growth conditions were 30–37 °C, pH 7.0–7.4 and 0.17 M NaCl. The G+C content of the genome of strain YSF-03 was 41.3 mol%. Phylogenetic analysis revealed that strain YSF-03 was most closely related to Methanolobus profundi MobM (98.15 % similarity in 16S rRNA gene sequence). Genome relatedness between strain YSF-03 and MobM was computed using the Genome-to-Genome Distance Calculator and average nucleotide identity, which gave values of 23.5 and 79.3 %, respectively. Based on the morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain YSF-03 represents a novel species of the genus Methanolobus , for which the name Methanolobus psychrotolerans sp. nov. is proposed. The type strain is YSF-03 (=BCRC AR10049=DSM 104044=NBRC 112514).

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2018-03-06
2019-12-06
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