1887

Abstract

Four bacterial strains recovered from shellfish (n=3) and from the water (n=1) of a canal contaminated with urban sewage were recognized as belonging to a novel species of the genus Arcobacter (represented by strain F138-33) by using a polyphasic characterization. All the new isolates required 2 % NaCl to grow. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all strains clustered together, with the most closely related species being Arcobacter marinus and Arcobacter molluscorum . However, phylogenetic analyses using the concatenated sequences of housekeeping genes (atpA, gyrB, hsp60, gyrA and rpoB) showed that all the novel strains formed a distinct lineage within the genus Arcobacter . Results of in silico DNA–DNA hybridization and the average nucleotide identity between the genome of strain F138-33 and those of the closely related species A. marinus and other relatively closely related species such as A. molluscorum and Arcobacter halophilus were all below 70 and 96 %, respectively. All the above results, together with the 15 physiological and biochemical tests that could distinguish the newly isolated strains from the closely related species, confirmed that these strains represent a novel species for which the name Arcobacter canalis sp. nov. is proposed, with the type strain F138-33 (=CECT 8984=LMG 29148).

Keyword(s): 16S rRNA , A. canalis , ANI , Arcobacter , isDDH , MLPA and shellfish
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2018-02-28
2019-10-23
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