1887

Abstract

A rod-shaped, Gram-stain-positive, motile and aerobic bacterium, designated LM2416, was isolated from faeces of living in Seoul Grand Park, Gyeonggi-do, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain LM2416 belonged to the genus , sharing high 16S rRNA gene sequence similarities to LMG 19488 (99.0 %), LMG 20964 (98.4 %), Hal 1 (98.3 %) and S1-20 (97.9 %). The isolate grew at 10–30 °C, pH 6–7 and 0–20 % (w/v) NaCl. Optimal growth was observed at 30 °C, pH 6–7 and 10 % (w/v) NaCl. The major fatty acid was anteiso-C. Polar lipids were composed of phosphatidylglycerol, diphosphatidylglycerol, three unknown phospholipids and two unknown aminophospholipids. The main menaquinone was MK-7. Strain LM2416 had alanine, lysine, glutamic acid, glycine and aspartic acid as cell-wall amino acids and ribose as a cell-wall sugar. The whole genome sequences of strain LM2416 and KCTC 3820 were sequenced by PacBio RS II sequencing. The genome sequence-based G+C content of strain LM2416 was 39.5 mol%. The orthologous average nucleotide identity value, showing genetic relatedness between strain LM2416 and KCTC 3820, was 78.3 %. Based on the phylogenetic, biochemical, chemotaxonomic and genotypic data presented in this study, strain LM2416 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is LM2416 (=KCTC 33927=JCM 32144).

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2018-04-01
2020-01-29
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