1887

Abstract

A strain of a novel anaerobic, Gram-stain-negative coccus was isolated from the tongue biofilm of a Thai child. This strain was shown, at the phenotypic level and based on 16S rRNA gene sequencing, to be a member of the genus . Comparative analysis of the 16S rRNA, and gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus . The novel strain showed 99.8, 95.1 and 95.9 % similarity to partial 16S rRNA, and gene sequences, respectively, to the type strains of the two most closely related species, ATCC 17748 and ATCC BAA-2400. The novel strain could be discriminated from previously reported species of the genus based on partial and gene sequencing and average nucleotide identity values. The major acid end-product produced by this strain was acetic acid under anaerobic conditions in trypticase-yeast extract-haemin with 1 % (w/v) glucose or fructose medium. Lactate was fermented to acetic acid and propionic acid. Based on these observations, this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is T11011-4 (=JCM 31738=TSD-88).

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2018-04-01
2019-12-12
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