1887

Abstract

A taxonomic study was carried out on strain LW15, which was isolated from the external lesions of diseased farmed Murray cod (Maccullochella peelii peelii) from an intensive culture pond. Cells of strain LW15 were Gram-negative, facultative-anaerobic, non-motile, and both coccobacillus- and bacillus-shaped. Growth was observed at NaCl concentrations of 0–2 % (w/v) (optimum, 0 %), 4–32 °C (optimum, 25–28 °C) and pH 5.0–9.0 (optimum, 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LW15 was affiliated to the genus Acinetobacter , showing the highest similarity to Acinetobacter guillouiae CIP 63.46 (97.7 %) and other Acinetobacter species with validly published names (93.5–97.6 %). Whole-genome sequencing and phylogeny reconstruction based on a core set of 1061 Acinetobacter genes indicated that strain LW15 was most closely related to the clade formed by A. guillouiae CIP 63.46 and Acinetobacter bereziniae CIP 70.12 and distantly related to any of the described species of genus Acinetobacter . Furthermore, strain LW15 could be distinguished from all known Acinetobacter species by its ability to assimilate β-alanine and l-arginine, but not d-glucose. The principal fatty acids were C18 : 1ω9c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The major respiratory quinone was Q-9. Polar lipids of strain LW15 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four phospholipids, aminolipid and two unknown lipids. Based on its phenotypic and genotypic data, strain LW15 represents a novel species of the genus Acinetobacter , for which the name Acinetobacter piscicola sp. nov. is proposed. The type strain is LW15 (=MCCC 1K03337=CICC 24241=KCTC 62134=JCM 32101).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002608
2018-01-29
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/3/905.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002608&mimeType=html&fmt=ahah

References

  1. Brisou J, Prevot AR. Studies on bacterial taxonomy. X. The revision of species under Acromobacter group. Ann Inst Pasteur 1954; 86: 722– 728 [PubMed]
    [Google Scholar]
  2. Touchon M, Cury J, Yoon EJ, Krizova L, Cerqueira GC et al. The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences. Genome Biol Evol 2014; 6: 2866– 2882 [CrossRef] [PubMed]
    [Google Scholar]
  3. Nemec A, Musílek M, Maixnerová M, de Baere T, van der Reijden TJ et al. Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans. Int J Syst Evol Microbiol 2009; 59: 118– 124 [CrossRef] [PubMed]
    [Google Scholar]
  4. Nemec A, Radolfova-Krizova L, Maixnerova M, Vrestiakova E, Jezek P et al. Taxonomy of haemolytic and/or proteolytic strains of the genus Acinetobacter with the proposal of Acinetobacter courvalinii sp. nov. (genomic species 14 sensu Bouvet & Jeanjean), Acinetobacter dispersus sp. nov. (genomic species 17), Acinetobacter modestus sp. nov., Acinetobacter proteolyticus sp. nov. and Acinetobacter vivianii sp. nov. Int J Syst Evol Microbiol 2016; 66: 1673– 1685 [CrossRef] [PubMed]
    [Google Scholar]
  5. Nock CJ, Elphinstone MS, Rowland SJ, Baverstock PR. Phylogenetics and revised taxonomy of the Australian freshwater cod genus, Maccullochella (Percichthyidae). Mar Freshw Res 2010; 61: 980– 991 [CrossRef]
    [Google Scholar]
  6. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester, UK: John Wiley and Sons; 1991; pp. 115– 175
    [Google Scholar]
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33: 1870– 1874 [CrossRef] [PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [CrossRef] [PubMed]
    [Google Scholar]
  9. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20: 265– 272 [CrossRef] [PubMed]
    [Google Scholar]
  10. Chan JZ, Halachev MR, Loman NJ, Constantinidou C, Pallen MJ. Defining bacterial species in the genomic era: insights from the genus Acinetobacter. BMC Microbiol 2012; 12: 1– 11 [CrossRef] [PubMed]
    [Google Scholar]
  11. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106: 19126– 19131 [CrossRef] [PubMed]
    [Google Scholar]
  12. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14: 60 [CrossRef] [PubMed]
    [Google Scholar]
  13. Graham PH, Sadowsky MJ, Keyser HH, Barnet YM, Bradley RS et al. Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int J Syst Bacteriol 1991; 41: 582– 587 [CrossRef]
    [Google Scholar]
  14. Wayne L, Wayne LG, Brenner DJ, Colwell RR, Grimont PAD et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [Crossref]
    [Google Scholar]
  15. Coram NJ, Rawlings DE. Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 °C. Appl Environ Microbiol 2002; 68: 838– 845 [CrossRef] [PubMed]
    [Google Scholar]
  16. Tønjum T, Welty DB, Jantzen E, Small PL. Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum: mapping of their relationships to M. tuberculosis by fatty acid profile analysis, DNA-DNA hybridization, and 16S rRNA gene sequence analysis. J Clin Microbiol 1998; 36: 918– 925 [PubMed]
    [Google Scholar]
  17. Poppel MT, Skiebe E, Laue M, Bergmann H, Ebersberger I et al. Acinetobacter equi sp. nov., isolated from horse faeces. Int J Syst Evol Microbiol 2016; 66: 881– 888 [CrossRef] [PubMed]
    [Google Scholar]
  18. Smibert RM, Krieg NR. Methods for General and Molecular Bacteriology American Society for Microbiology; 1994; pp. 647– 654
    [Google Scholar]
  19. Cruze J, Singer J, Finnerty W. Conditions for quantitative transformation in Acinetobacter calcoaceticus. Curr Microbiol 1979; 3: 129– 132 [Crossref]
    [Google Scholar]
  20. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009; 59: 2114– 2121 [CrossRef] [PubMed]
    [Google Scholar]
  21. Liu Y, Shang XX, Yi ZW, Gu L, Zeng RY. Shewanella mangrovi sp. nov., an acetaldehyde-degrading bacterium isolated from mangrove sediment. Int J Syst Evol Microbiol 2015; 65: 2630– 2634 [CrossRef] [PubMed]
    [Google Scholar]
  22. Krizova L, Maixnerova M, Sedo O, Nemec A. Acinetobacter albensis sp. nov., isolated from natural soil and water ecosystems. Int J Syst Evol Microbiol 2015; 65: 3905– 3912 [CrossRef] [PubMed]
    [Google Scholar]
  23. Minnikin DE, Odonnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [Crossref]
    [Google Scholar]
  24. Dahal RH, Chaudhary DK, Kim J. Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil. Arch Microbiol 2017; 199: 701– 710 [CrossRef] [PubMed]
    [Google Scholar]
  25. Liu S, Wang Y, Ruan Z, Ma K, Wu B et al. Acinetobacter larvae sp. nov., isolated from the larval gut of Omphisa fuscidentalis. Int J Syst Evol Microbiol 2017; 67: 806– 811 [CrossRef] [PubMed]
    [Google Scholar]
  26. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 1987; 19: 161– 207 [Crossref]
    [Google Scholar]
  27. Juni E. Genus II. Acinetobacter Brisou and Prévot 1954,727AL. In Brenner DJ, Krieg NR, Stanley JT. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol. 2B New York: Springer; 2005; pp. 425– 437
    [Google Scholar]
  28. Nemec A, Radolfova-Krizova L, Maixnerova M, Sedo O. Acinetobacter colistiniresistens sp. nov. (formerly genomic species 13 sensu Bouvet and Jeanjean and genomic species 14 sensu Tjernberg and Ursing), isolated from human infections and characterized by intrinsic resistance to polymyxins. Int J Syst Evol Microbiol 2017; 67: 2134– 2141 [CrossRef] [PubMed]
    [Google Scholar]
  29. Hu Y, Feng Y, Zhang X, Zong Z. Acinetobacter defluvii sp. nov., recovered from hospital sewage. Int J Syst Evol Microbiol 2017; 67: 1709– 1713 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002608
Loading
/content/journal/ijsem/10.1099/ijsem.0.002608
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error