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Abstract

A novel strictly anaerobic bacterium, designated strain BA2-13, was isolated from a thermophilic industrial-scale biogas plant. Cells were rod-shaped and Gram-stain-positive. Growth occurred at temperatures of 25 to 50 °C and between pH 6.3 and 9.5. Strain BA2-13 produced indole. Cell growth was stimulated by yeast extract, peptone, meat extract, a mixture of 20 amino acids, glucose, pyruvate and ribose. When grown on peptone and yeast extract, the main fermentation products were acetic acid, H2 and CO2. The predominant cellular fatty acids were iso-C15 : 0 and iso-C14 : 0 3-OH. Major polar lipids were diphosphatidylglycerol, glycolipids, phospholipids and phosphatidylgycerol. Phylogenetic analysis based on 16S rRNA gene nucleotide sequence analysis placed strain BA2-13 within the order Clostridiales showing closest affiliation with Proteiniborus ethanoligenes with 95.9 % sequence identity. Physiological, genotypic and chemotaxonomic differences of strain BA2-13 from P. ethanoligenes support the description of a new species within the genus Proteiniborus for which we suggest the name Proteiniborus indolifex sp. nov. (type strain BA2-13=DSM 103060=LMG 29818).

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2018-01-25
2019-10-19
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