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Volume 68, Issue 3

sp. nov., isolated from a thermophilic industrial-scale biogas plant Free

Abstract

A novel strictly anaerobic bacterium, designated strain BA2-13, was isolated from a thermophilic industrial-scale biogas plant. Cells were rod-shaped and Gram-stain-positive. Growth occurred at temperatures of 25 to 50 °C and between pH 6.3 and 9.5. Strain BA2-13 produced indole. Cell growth was stimulated by yeast extract, peptone, meat extract, a mixture of 20 amino acids, glucose, pyruvate and ribose. When grown on peptone and yeast extract, the main fermentation products were acetic acid, H and CO. The predominant cellular fatty acids were iso-C and iso-C 3-OH. Major polar lipids were diphosphatidylglycerol, glycolipids, phospholipids and phosphatidylgycerol. Phylogenetic analysis based on 16S rRNA gene nucleotide sequence analysis placed strain BA2-13 within the order showing closest affiliation with with 95.9 % sequence identity. Physiological, genotypic and chemotaxonomic differences of strain BA2-13 from support the description of a new species within the genus for which we suggest the name sp. nov. (type strain BA2-13=DSM 103060=LMG 29818).

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Keyword(s): anaerobic digestion , biomethanation , Clostridia , Firmicutes , maize silage and manure
© 2018 IUMS
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2018-03-01
2024-05-10
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Proteiniborus indolifex sp. nov., isolated from a thermophilic industrial-scale biogas plant
Int J Syst Evol Microbiol 68, 824 (2018); https://doi.org/10.1099/ijsem.0.002591
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