1887

Abstract

Three bacterial strains, HKU63, HKU64 and HKU65, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong. The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species. 16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity. Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63 and HKU64, 94.2–97.0 % nucleotide sequence identities between HKU63/HKU64 and HKU65 and the three strains shared 82.9–98.9 % sequence identities with other currently recognized Tsukamurella species. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (23.0±4.2 to 50.7±3.7 % DNA–DNA relatedness), of which HKU63 and HKU64 represented the same species (≥95.2±4.8 % DNA–DNA relatedness) while HKU65 represented another species. Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content of strains HKU63, HKU64 and HKU65 were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively. A novel species, Tsukamurella ocularis sp. nov. is proposed to accommodate strains HKU63 and HKU64, with HKU63 (=JCM 31969=DSM 105034) designated as the type strain whilst another novel species, Tsukamurella hominis sp. nov., is proposed to accommodate the third strain, HKU65, which is designated as the type strain (=JCM 31971=DSM 105036).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002589
2018-02-05
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/3/810.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002589&mimeType=html&fmt=ahah

References

  1. Collins MD, Smida J, Dorsch M, Stackebrandt E. Tsukamurella gen. nov. harboring Corynebacterium paurometabolum and Rhodococcus aurantiacus. Int J Syst Bacteriol 1988;38:385–391 [CrossRef]
    [Google Scholar]
  2. Olson JB, Harmody DK, Bej AK, McCarthy PJ. Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marine sponge. Int J Syst Evol Microbiol 2007;57:1478–1481 [CrossRef][PubMed]
    [Google Scholar]
  3. Park SW, Kim SM, Park ST, Kim YM. Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete. Int J Syst Evol Microbiol 2009;59:1541–1544 [CrossRef][PubMed]
    [Google Scholar]
  4. Seong CN, Kim YS, Baik KS, Choi SK, Kim MB et al. Tsukamurella sunchonensis sp. nov., a bacterium associated with foam in activated sludge. J Microbiol 2003;41:83–88
    [Google Scholar]
  5. Yassin AF, Rainey FA, Brzezinka H, Burghardt J, Rifai M et al. Tsukamurella pulmonis sp. nov. Int J Syst Bacteriol 1996;46:429–436 [CrossRef][PubMed]
    [Google Scholar]
  6. Yassin AF, Rainey FA, Burghardt J, Brzezinka H, Schmitt S et al. Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol 1997;47:607–614 [CrossRef][PubMed]
    [Google Scholar]
  7. Nam SW, Kim W, Chun J, Goodfellow M. Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 2004;54:1209–1212 [CrossRef][PubMed]
    [Google Scholar]
  8. Teng JL, Tang Y, Huang Y, Guo FB, Wei W et al. Phylogenomic analyses and reclassification of species within the genus Tsukamurella: insights to species definition in the post-genomic era. Front Microbiol 2016;7:1137 [CrossRef][PubMed]
    [Google Scholar]
  9. Oren A, Garrity GM. Notification of changes in taxonomic opinion previously published outside the IJSEM. Int J Syst Evol Microbiol 2017;67:2081–2086 [CrossRef][PubMed]
    [Google Scholar]
  10. Bouza E, Pérez-Parra A, Rosal M, Martín-Rabadán P, Rodríguez-Créixems M et al. Tsukamurella: a cause of catheter-related bloodstream infections. Eur J Clin Microbiol Infect Dis 2009;28:203–210 [CrossRef][PubMed]
    [Google Scholar]
  11. Liu CY, Lai CC, Lee MR, Lee YC, Huang YT et al. Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates. Int J Antimicrob Agents 2011;38:534–537 [CrossRef][PubMed]
    [Google Scholar]
  12. Schwartz MA, Tabet SR, Collier AC, Wallis CK, Carlson LC et al. Central venous catheter-related bacteremia due to Tsukamurella species in the immunocompromised host: a case series and review of the literature. Clin Infect Dis 2002;35:e72-77 [CrossRef][PubMed]
    [Google Scholar]
  13. Woo PC, Fong AH, Ngan AH, Tam DM, Teng JL et al. First report of Tsukamurella keratitis: association between T. tyrosinosolvens and T. pulmonis and ophthalmologic infections. J Clin Microbiol 2009;47:1953–1956 [CrossRef][PubMed]
    [Google Scholar]
  14. Woo PC, Ngan AH, Lau SK, Yuen KY. Tsukamurella conjunctivitis: a novel clinical syndrome. J Clin Microbiol 2003;41:3368–3371 [CrossRef][PubMed]
    [Google Scholar]
  15. Nam SW, Chun J, Kim S, Kim W, Zakrzewska-Czerwinska J et al. Tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants. Syst Appl Microbiol 2003;26:367–375 [CrossRef][PubMed]
    [Google Scholar]
  16. Maeda Y, Stanley T, Stirling J, Griffiths M, Calvert A et al. No evidence of transmission of bacteria between reptiles and a CF patient–a case report of a young adult CF patient and reptiles. Zoonoses Public Health 2010;57:e47-53 [CrossRef][PubMed]
    [Google Scholar]
  17. Jiang Y, Chen X, Han L, Qy L, Huang XS et al. Diversity of cultivable actinomycetes in 6 species of herbivore feces. Int J Microbiol Res 2013;1:76–84
    [Google Scholar]
  18. Tang Y, Teng JL, Cheung CL, Ngan AH, Huang Y et al. Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra). Int J Syst Evol Microbiol 2016;66:3329–3336 [CrossRef][PubMed]
    [Google Scholar]
  19. To KK, Fung AM, Teng JL, Curreem SO, Lee KC et al. Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting. J Clin Microbiol 2013;51:334–338 [CrossRef][PubMed]
    [Google Scholar]
  20. Teng JL, Tang Y, Wong SS, Ngan AH, Huang Y et al. Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis. Int J Syst Evol Microbiol 2016;66:391–397 [CrossRef][PubMed]
    [Google Scholar]
  21. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.611–651
    [Google Scholar]
  22. Kattar MM, Cookson BT, Carlson LD, Stiglich SK, Schwartz MA et al. Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. J Clin Microbiol 2001;39:1467–1476 [CrossRef][PubMed]
    [Google Scholar]
  23. Lau SK, Curreem SO, Lin CC, Fung AM, Yuen KY et al. Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish. Int J Syst Evol Microbiol 2013;63:2570–2576 [CrossRef][PubMed]
    [Google Scholar]
  24. Lau SK, McNabb A, Woo GK, Hoang L, Fung AM et al. Catabacter hongkongensis gen. nov., sp. nov., isolated from blood cultures of patients from Hong Kong and Canada. J Clin Microbiol 2007;45:395–401 [CrossRef][PubMed]
    [Google Scholar]
  25. Woo PC, Fung AM, Lau SK, Chan BY, Chiu SK et al. Granulicatella adiacens and Abiotrophia defectiva bacteraemia characterized by 16S rRNA gene sequencing. J Med Microbiol 2003;52:137–140 [CrossRef][PubMed]
    [Google Scholar]
  26. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 1999;41:95–98
    [Google Scholar]
  27. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  28. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  29. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  30. Abramoff MD, Magalhães PJ, Ram SJ. Image processing with ImageJ. Biophotonics international 2004;11:36–42
    [Google Scholar]
  31. Sasser M. Identification of bacteria through fatty acid analysis. In Klement Z, Rudolph K, Sands DC. (editors) Methods in Phytobacteriology Budapest: Akademiai Kiado; 1990; pp.199–204
    [Google Scholar]
  32. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  33. Rhuland LE, Work E, Denman RF, Hoare DS. The Behavior of the Isomers of α,ε-Diaminopimelic Acid on Paper Chromatograms. J Am Chem Soc 1955;77:4844–4846 [CrossRef]
    [Google Scholar]
  34. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  35. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TMS et al. (editors) Methods for General and Molecular Microbiology Washington, DC: American Society for Microbiology; 2007; pp.330–393
    [Google Scholar]
  36. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962;5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  37. Woo PC, Tse H, Lau SK, Leung KW, Woo GK et al. Alkanindiges hongkongensis sp. nov. a novel Alkanindiges species isolated from a patient with parotid abscess. Syst Appl Microbiol 2005;28:316–322 [CrossRef][PubMed]
    [Google Scholar]
  38. Lechevalier MP, de Bievre C, Lechevalier H. Chemotaxonomy of aerobic Actinomycetes: phospholipid composition. Biochem Syst Ecol 1977;5:249–260 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002589
Loading
/content/journal/ijsem/10.1099/ijsem.0.002589
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error