1887

Abstract

A novel rod-shaped, Gram-stain-negative, aerobic bacterium, designated S35, was isolated from deep-sea sediment collected from the Pacmanus hydrothermal field, Manus Basin, Papua New Guinea. Strain S35 grew optimally at 28 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain S35 shared 97.38–98.55% similarity with the type strains of Alteromonas lipolytica, Alteromonas mediterranea and Aestuariibacter halophilus . Phylogenetic analysis showed that strain S35 belonged to the genus Alteromonas. The strain contained ubiquinone-8 as the predominant respiratory lipoquinone. Summed feature 3 (C16 : 1ω7c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 were the major fatty acids. The DNA G+C content of strain S35 was 51.3 mol%. These results indicated that strain S35 represents a novel species of the genus Alteromonas, for which the name Alteromonas oceani sp. nov. (type strain S35=KCTC 52449=CGMCC 1.16029) is proposed.

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2018-01-09
2019-12-11
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References

  1. Baumann L, Baumann P, Mandel M, Allen RD. Taxonomy of aerobic marine eubacteria. J Bacteriol 1972; 110: 402– 429 [PubMed]
    [Google Scholar]
  2. Baumann P, Baumann L, Bowditch RD, Beaman B. Taxonomy of Alteromonas: A. nigrifaciens sp. nov., nom. rev.; A. macleodii; and A. haloplanktis. Int J Syst Bacteriol 1984; 34: 145– 149 [CrossRef]
    [Google Scholar]
  3. Chen YG, Xiao HD, Tang SK, Zhang YQ, Borrathybay E et al. Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 2009; 96: 259– 266 [CrossRef] [PubMed]
    [Google Scholar]
  4. Jin HM, Kim KH, Jeon CO. Alteromonas naphthalenivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from tidal-flat sediment. Int J Syst Evol Microbiol 2015; 65: 4208– 4214 [CrossRef] [PubMed]
    [Google Scholar]
  5. Martínez-Checa F, Béjar V, Llamas I, del Moral A, Quesada E. Alteromonas hispanica sp. nov., a polyunsaturated-fatty-acid-producing, halophilic bacterium isolated from Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 2005; 55: 2385– 2390 [CrossRef] [PubMed]
    [Google Scholar]
  6. Yoon JH, Kim IG, Kang KH, Oh TK, Park YH. Alteromonas marina sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 2003; 53: 1625– 1630 [CrossRef] [PubMed]
    [Google Scholar]
  7. Yoon JH, Yeo SH, Oh TK, Park YH. Alteromonas litorea sp. nov., a slightly halophilic bacterium isolated from an intertidal sediment of the Yellow Sea in Korea. Int J Syst Evol Microbiol 2004; 54: 1197– 1201 [CrossRef] [PubMed]
    [Google Scholar]
  8. Matsuyama H, Minami H, Sakaki T, Kasahara H, Baba S et al. Alteromonas gracilis sp. nov., a marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 2015; 65: 1498– 1503 [CrossRef] [PubMed]
    [Google Scholar]
  9. Ivanova EP, Bowman JP, Lysenko AM, Zhukova NV, Gorshkova NM et al. Alteromonas addita sp. nov. Int J Syst Evol Microbiol 2005; 55: 1065– 1068 [CrossRef] [PubMed]
    [Google Scholar]
  10. Ivanova EP, Ng HJ, Webb HK, Kurilenko VV, Zhukova NV et al. Alteromonas australica sp. nov., isolated from the Tasman Sea. Antonie Van Leeuwenhoek 2013; 103: 877– 884 [CrossRef] [PubMed]
    [Google Scholar]
  11. Park S, Kang CH, Won SM, Park JM, Kim BC et al. Alteromonas confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2015; 65: 3603– 3608 [CrossRef] [PubMed]
    [Google Scholar]
  12. van Trappen S, Tan TL, Yang J, Mergaert J, Swings J. Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas. Int J Syst Evol Microbiol 2004; 54: 1157– 1163 [CrossRef] [PubMed]
    [Google Scholar]
  13. Ivanova EP, López-Pérez M, Zabalos M, Nguyen SH, Webb HK et al. Ecophysiological diversity of a novel member of the genus Alteromonas, and description of Alteromonas mediterranea sp. nov. Antonie van Leeuwenhoek 2015; 107: 119– 132 [CrossRef] [PubMed]
    [Google Scholar]
  14. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acids Techniques in Bacterial Systematics New York: John Wiley and Sons Inc; 1991; pp. 115– 147
    [Google Scholar]
  15. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62: 716– 721 [CrossRef] [PubMed]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406– 425 [PubMed]
    [Google Scholar]
  17. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol 1992; 9: 945– 967
    [Google Scholar]
  18. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 2003; 52: 696– 704 [CrossRef] [PubMed]
    [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16: 111– 120 [CrossRef] [PubMed]
    [Google Scholar]
  21. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070
    [Google Scholar]
  22. Sun QL, Sun L. Description of Domibacillus iocasae sp. nov., isolated from deep-sea sediment, and emended description of the genus Domibacillus. Int J Syst Evol Microbiol 2016; 66: 982– 987 [CrossRef] [PubMed]
    [Google Scholar]
  23. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3: 208– IN1 [CrossRef]
    [Google Scholar]
  24. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39: 159– 167 [CrossRef]
    [Google Scholar]
  25. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 1990; 20: 16
    [Google Scholar]
  26. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2: 233– 241 [CrossRef]
    [Google Scholar]
  27. López-Pérez E, Rodriguez-Valera F. The family Alteromonadaceae. The Prokaryotes-Gammaproteobacteria, 4th ed. 2014; pp. 69– 92
    [Google Scholar]
  28. Shi XL, Wu YH, Jin XB, Wang CS, Xu XW. Alteromonas lipolytica sp. nov., isolated from the surface seawater. Int J Syst Evol Microbiol 2017; 67: 237– 242 [Crossref]
    [Google Scholar]
  29. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37: 463– 464 [Crossref]
    [Google Scholar]
  30. Yi H, Bae KS, Chun J. Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii. Int J Syst Evol Microbiol 2004; 54: 571– 576 [CrossRef] [PubMed]
    [Google Scholar]
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