1887

Abstract

A Gram-stain-negative, facultatively aerobic, motile-by-gliding, non-flagellated and rod-shaped bacterial strain, designated YSM-43, was isolated from a tidal flat in Yeosu on the South Sea in the Republic of Korea. Strain YSM-43 grew optimally at 30 °C and in the presence of 1.0–2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain YSM-43 fell within the clade comprising type strains of Flavobacterium species, clustering with the type strains of Flavobacterium jejuense and Flavobacterium jumunjinense . It exhibited 16S rRNA gene sequence similarity values of 97.20 and 97.15 % to the type strains of F. jejuense and F. jumunjinense , respectively, and of less than 96.59 % to the type strains of the other Flavobacterium species. Strain YSM-43 contained menaquinone-6 as the predominant menaquinone and iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G and iso-C15 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain YSM-43 was 29.8 mol% and its DNA–DNA relatedness values with type strains of F. jejuense and F. jumunjinense were 13 and 11 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic data, revealed that strain YSM-43 is separate from other recognized species of the genus Flavobacterium . On the basis of the data presented, strain YSM-43 is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium sediminilitoris sp. nov. is proposed. The type strain is YSM-43 (=KACC 19435=KCTC 62142=NBRC 113020).

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2018-01-05
2019-10-19
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