1887

Abstract

A Gram-stain-positive, non-motile, yellow and rod-shaped actinobacterium, designated strain Brt-A, isolated from oil-contaminated soil, grew at 15–40 °C, at pH 5.5–10.0 and at 0–2 % (w/v) NaCl concentration. This strain was characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain Brt-A belonged to the genus and is closely related to YIM 101269, SST-39, LNB-140 and RP1 (99.03, 97.00, 96.88, and 96.46 % gene sequence similarity, respectively). The predominant respiratory quinone was MK-9(H); the major polar lipids were phosphatidylglycerol and diphosphatidylglycerol; the predominant polyamines were spermine and spermidine; and the major fatty acids were anteiso-C and iso-C. The cell-wall peptidoglycan contained -diaminopimelic acid; and glucose and ribose were detected as diagnostic sugars from whole-cell hydrolysates. The DNA G+C content was 68.1 mol%. The DNA–DNA relatedness between strain Brt-A and its closely related species of the genus were between 55.0–44.0 %, which fall below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain Brt-A represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Brt-A (=KEMB 9005-690=KACC 19391=JCM 32157).

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2018-02-01
2020-01-26
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