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Abstract

Two isolates of bacterial strains A15 and A17 were isolated from stream bank soil in Kyonggi University. Cells were aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, non-spore-forming, rod-shaped, opaque, and cream coloured. Both strains hydrolysed CM-cellulose. Strains were able to grow at 20–42 °C, pH 5.5–10.0 and at 1.5 % NaCl concentration (w/v). Indole test was positive. Analyses of phylogenetic trees based on its 16S rRNA gene sequences indicated that strain A15 formed a lineage within the family Rhodospirillaceae of the phylum Proteobacteria which was distinct from Ferrovibrio denitrificans S3 (98.4 % sequence similarity) and Ferrovibrio xuzhouensis LM-6 (97.4 %). The sole detected respiratory quinone was Q–10. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The major cellular fatty acids were C19 : 0 cycloω8c, C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C18 : 0cyclo and C12 : 0. The DNA G+C contents of strains A15 and A17 were 63.4 and 62.9 mol%, respectively. DNA–DNA relatedness between strain A15 and other two members of the genus Ferrovibrio ranged from 25 to 37 %. The polyphasic characterization revealed strains A15 and A17 represent a novel species in the genus Ferrovibrio , for which the name Ferrovibrio soli sp. nov. is proposed. The type strain is A15 (=KEMB 9005-522=KACC 19102=NBRC 112682).

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2017-12-08
2019-10-19
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