1887

Abstract

A novel marine Gram-negative bacterium, designated strain GH1-23, was isolated from a tidal mudflat sample collected at Dongmak seashore on Gangwha Island, Republic of Korea and its taxonomic position was determined through a polyphasic investigation. The bacterium was strictly aerobic, chemoheterotrophic, catalase- and oxidase-positive, consisted of non-motile rods and grew optimally at 30 °C, pH 7 and 1 % NaCl. The predominant cellular fatty acids were C18 : 1 ω7c and cyclo-C19 : 0 ω8c. The major isoprenoid quinone was Q-10. The major polar lipids were phosphatidylglycerol, a phosphoglycolipid and an aminolipid. Comparative 16S rRNA gene sequence analysis revealed that the isolate was closely related to members of the genus Maribius . The highest 16S rRNA gene sequence similarity was found to be 97.5 % to Maribius salinus followed by 97.4 % to Maribius pelagius ; levels of 16S rRNA gene sequence similarity between the novel strain and other representatives of family Rhodobacteraceae were <95.5 %. The DNA G+C content was 66.7 mol % and DNA–DNA relatedness values with the type strains of species of the genus Maribius were 33–39 %. Based on combined data from a polyphasic investigation, strain GH1-23 (=KCTC 52957=DSM 104950) represents a novel species of the genus Maribius , for which the name Maribius pontilimi sp. nov. is proposed.

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2017-12-05
2019-10-23
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