1887

Abstract

A novel actinomycete strain PS42-9, which formed short chains of spores borne on the tip of long sporophores arising from the substrate mycelium, was isolated from soil in Phu-Sang National Park, Phayao province, Thailand. The isolate contained -diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain PS42-9 were glucose, madurose, mannose, rhamnose and ribose. The characteristic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive glycophospholipids. The major menaquinone was MK-10(H). The main cellular fatty acids were Cω8 and C. The G+C content of the genomic DNA was 71.5 mol%. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain PS42-9 should be classified in the genus and was closely related to DMKUA 205 (99.10 %) and NBRC 106371 (98.61 %). The result of DNA–DNA hybridization and some physiological and biochemical properties indicated that strain PS42-9 could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is PS42-9 (=BCC 46909=NBRC 108780).

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2018-01-01
2021-10-24
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