1887

Abstract

Two actinobacterial isolates, strains SG15 and SGB14, were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora . For strains SG15 and SGB14, the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248 (99.2 %) and Micromonospora purpureochromogenes DSM 43821 (99.4 %), respectively. However, strains SG15 and SGB14 were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15 (=CECT 9369; =DSM 105363), and Micromonospora luteiviridis sp. nov. type strain SGB14 (=CECT 9370; =DSM 105362).

Keyword(s): endophyte , Micromonospora , MLSA , nodule , Pisum and taxonomy
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2017-11-17
2019-10-14
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