1887

Abstract

An actinomycete strain, designated KLBMP S0039, was isolated from the rhizosphere soil of Linn., collected from the coastal region in Lianyungang, Jiangsu Province, eastern PR China, and was studied to determine its taxonomic position. The isolate showed a combination of morphological and chemotaxonomic properties typical of the members of the genus . The cell wall contained -diaminopimelic acid as the diagnostic diamino acid and the whole-cell sugars were galactose, arabinose, glucose and ribose. The predominant menaquinone was identified as MK-8(Hω-cycl). The diagnostic phospholipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unknown lipids. The predominant cellular fatty acids were identified as C, C, Cω9, and 10-methyl C [tuberculostearic acid (TBSA)]. The G+C content of the genomic DNA was determined to be 68.2 mol%. The 16S rRNA gene sequence similarity indicated that KLBMP S0039 was most closely related to NBRC 108232 (99.4 % 16S rRNA gene sequence similarity) and NBRC 15531 (99.2 %), similarities to other type strains of species of the genus were found to be less than 98.6 %. However, DNA–DNA relatedness values and phenotypic data indicated that KLBMP S0039 could be clearly distinguished from the closely related species of the genus . On the basis of polyphasic taxonomic data, it is concluded that KLBMP S0039 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KLBMP S0039 (=KCTC 39693=CGMCC 4.7329).

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2018-01-01
2024-12-05
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