1887

Abstract

A novel endophytic bacterium, designated strain HZ7, was isolated from the root nodules of growing in a lead–zinc mine in Mianxian County, Shaanxi Province, China. Cells were Gram-reaction-negative, aerobic, motile, rod-shaped, methyl-red-negative, catalase-positive, positive for chitosan-degrading activity and did not produce HS. Strain HZ7 grew at 4–45 °C (optimum 25–30 °C), at pH 5–9 (optimum pH 7–8) and with 0–1 % (w/v) NaCl. The quinone type was ubiquinone 8 (UQ-8). The major fatty acids were identified as C, C cyclo and summed feature 3 (Cω7 and/or Cω6). The G+C content of the genomic DNA was 68.5 mol% by whole genome sequencing. According to 16S rRNA gene sequence analysis, the closest phylogenetic relative was 3001 (99.05 % similarity). Genome relatedness was computed using average nucleotide identity and genome-to-genome distance analysis, both of which strongly supported strain HZ7as belonging to the genus as a representative of a novel species. On the basis of phylogenetic analysis, chemotaxonomic data and physiological characteristics, strain HZ7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HZ7 (=JCM 31671=CCTCC AB 2014353).

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2018-01-01
2020-01-17
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