1887

Abstract

Two strains of bacteria, designated J107-1 and J107-2, were isolated from farmland soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, coccoid rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Both strains hydrolysed casein and tyrosine. Strains could grow at pH 9.0 and at 42 °C. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strains J107-1 and J107-2 formed a lineage within the phylum Bacteroidetes that was distinct from various members of the family Chitinophagaceae , including Ferruginibacter paludis HME8881 (89.71 % sequence similarity), Ferruginibacter yonginensis HME8442, Niastella yeongjuensis GR20-13 (89.70 %), Chitinophaga niabensis DSM 24787 (89.57 %), Lacibacter cauensis CGMCC 1.7271 (89.56 %) and Taibaiella koreensis THG-DT86 (89.52 %). The 16S rRNA gene similarities of other members of the family Chitinophagaceae are less than 89.51 %. The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C17 : 0. The DNA G+C content of strains ranged from 47.8 to 48.0 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strains J107-1 and J107-2 represent a novel genus in the family Chitinophagaceae, for which the name Rurimicrobium arvi gen. nov., sp. nov. is proposed. The type strain of Rurimicrobium arvi is J107-1 (=KEMB 9005-549=KACC 19167=JCM 31921).

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2017-10-26
2019-10-20
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