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Abstract

Strain KYPY9, isolated from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomy approach. Cells of KYPY9 were Gram-staining-negative, strictly aerobic, motile by means of a single polar flagellum, rod-shaped and formed light yellow colonies. Optimal growth occurred at 20–25 °C, at pH 7 and with 0 % NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that KYPY9 represented a member of the genus and the sequence similarity between the 16S rRNA genes of KYPY9 and of the type strains of other species of the genus ranged from 94.1 to 98.0 %. The closest relatives of KYPY9 were SHS5-24 (98.0 %) and CMJ-9 (97.0 %). KYPY9 had summed feature 3 (Cω7 and/or Cω6) and C as the predominant fatty acids. The major cellular hydroxy fatty acid was C 3-OH. KYPY9 had phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the predominant polar lipids. The polyamine profile was composed of the major compound 2-hydroxyputrescine and moderate amounts of putrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.4 mol%. The DNA–DNA relatedness of KYPY9 with respect to SHS5-24 and CMJ-9 was less than 35 %. On the basis of the phylogenetic inference and phenotypic data, KYPY9 was recognized as a representative of a novel species within the genus . The name sp. nov. is proposed, with KYPY9 (=BCRC 81009=LMG 29730=KCTC 52442) as the type strain.

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2017-12-01
2020-12-01
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