1887

Abstract

Three strains representing a novel species of yeast were isolated from samples of rotting wood collected from Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that the novel species is a member of the genus , although the formation of ascospores was not observed. The novel species was related most closely to the type strain of the species , but they had a 0.5 % sequence divergence (3 substitutions, 0 gap) in the D1/D2 domain and a 5.4 % sequence divergence (21 substitutions, 10 gaps) in the ITS region. The novel species could also be differentiated from the closely related species by some biochemical and physiological characteristics. The species name f.a., sp. nov. is proposed to accommodate these strains, with NYNU 16752 (=CICC 33163=CBS 14692) designated as the type strain. The MycoBank number is MB 822199.

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2017-12-01
2020-01-18
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References

  1. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 2008;8:939–954 [CrossRef][PubMed]
    [Google Scholar]
  2. Kurtzman CP. Lindnera Kurtzman, Robnett & Basehoar-Powers (2008). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed.vol. 2 San Diego: Elsevier; 2011; pp.521–543[Crossref]
    [Google Scholar]
  3. Minter DW. Cyberlindnera, a replacement name for Lindnera Kurtzman, et al., nom. Illegit. Mycotaxon 2009;110:473–476[Crossref]
    [Google Scholar]
  4. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP et al. Candida Berkhout (1923). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed.vol. 2 San Diego: Elsevier; 2011; pp.987–1278[Crossref]
    [Google Scholar]
  5. Chang CF, Liu YR, Chen SF, Naumov GI, Naumova ES et al. Five novel species of the anamorphic genus Candida in the Cyberlindnera clade isolated from natural substrates in Taiwan. Antonie van Leeuwenhoek 2012;102:9–21 [CrossRef][PubMed]
    [Google Scholar]
  6. Ninomiya S, Mikata K, Kajimura H, Kawasaki H. Two novel ascomycetous yeast species, Wickerhamomyces scolytoplatypi sp. nov. and Cyberlindnera xylebori sp. nov., isolated from ambrosia beetle galleries. Int J Syst Evol Microbiol 2013;63:2706–2711 [CrossRef][PubMed]
    [Google Scholar]
  7. Cadete RM, Cheab MA, Santos RO, Safar SV, Zilli JE et al. Cyberlindnera xylosilytica sp. nov., a xylitol-producing yeast species isolated from lignocellulosic materials. Int J Syst Evol Microbiol 2015;65:2968–2974 [CrossRef][PubMed]
    [Google Scholar]
  8. Guamán-Burneo MC, Dussán KJ, Cadete RM, Cheab MA, Portero P et al. Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov. Antonie van Leeuwenhoek 2015;108:919–931 [CrossRef][PubMed]
    [Google Scholar]
  9. Poomtien J, Jindamorakot S, Limtong S, Pinphanichakarn P, Thaniyavarn J. Two new anamorphic yeasts species, Cyberlindnera samutprakarnensis sp. nov. and Candida thasaenensis sp. nov., isolated from industrial wastes in Thailand. Antonie van Leeuwenhoek 2013;103:229–238 [CrossRef][PubMed]
    [Google Scholar]
  10. Zheng J, Liu KF, Liu XJ, Zhang L, Hui FL. Deakozyma yunnanensis sp. nov., a novel yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2017;67:2436–2439 [CrossRef][PubMed]
    [Google Scholar]
  11. Ren YC, Liu ST, Li Y, Hui FL. Pichia dushanensis sp. nov. and Hyphopichia paragotoi sp. nov., two sexual yeast species associated with insects and rotten wood. Int J Syst Evol Microbiol 2015;65:2875–2881 [CrossRef][PubMed]
    [Google Scholar]
  12. Wang Y, Ren YC, Li Y, Hui FL. Molecular phylogeny and taxonomy of Yamadazyma dushanensis f.a., sp. nov., a cellobiose-fermenting yeast species from China. Curr Microbiol 2015;71:268–273 [CrossRef][PubMed]
    [Google Scholar]
  13. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – a Taxonomic Study, 5th ed.vol. 1 Amsterdam: Elsevier; 2011; pp.87–110[Crossref]
    [Google Scholar]
  14. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  15. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: a Guide to Methods and Applications New York: Academic Press; 1990; pp.315–322
    [Google Scholar]
  16. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  17. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  20. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  21. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proc Natl Acad Sci USA 2012;109:6241–6246 [CrossRef][PubMed]
    [Google Scholar]
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