1887

Abstract

A novel bacterium, designated strain DL503, was isolated from a Daqu sample and its taxonomic position determined using a polyphasic taxonomy. Strain DL503 was a Gram-stain-negative, facultatively anaerobic, non-sporulating, motile and coccoid-rod-shaped bacterium. Optimum growth occurred at 20–45 °C, pH 5.0–10.0 and 1.5 % (w/v) NaCl. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus , showing highest levels of similarity with respect to JCM 16471 (98.94 %) and DSM 18396 (98.39 %). Cells contained the quinones Q-8 and MK-8, and the polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified polar lipids and three unidentified amino lipids. The DNA G+C content was 53.3 mol% and the major fatty acids were C, C cyclo, summed feature 3 (C ω7 and/or C ω6), summed feature 4 (C iso I and/or C anteiso B) and summed feature 8 (C ω7 and/or C ω6). The DNA–DNA relatedness values between strain DL503 and its close relatives, including JCM 16471 and DSM 18396, were 51.5±0.5 % and 45.2±1.1 %, respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DL503 (=LMG 29914=CGMCC 1.15944).

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2017-12-01
2024-11-04
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