1887

Abstract

A Gram-stain-negative, non-motile, aerobic and heterotrophic strain, designated 1-19b, was isolated from surface seawater in the Indian Ocean. The cells were ovoid or coccus (0.8–1.0 µm in diameter) with no flagellum. Activities of catalase and oxidase were positive. Growth was observed at salinity of 0.5–10 (%NaCl, w/v) with an optimum of 3–4, at pH 5–10 with an optimum of 7–8, and at 5–37 °C with an optimum of 28-35 °C. It accumulated poly-β-hydroxybutyrate granules inside the cell. Bacteriochlorophyll a was absent. The respiratory quinone was Q-10 and the dominant fatty acid was summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified phospholipid and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 1-19b belonged to the family Rhodobacteraceae and formed a distinct monophyletic clade with 'Oceaniovalibus guishaninsula' JLT2003 and Pontibaca methylaminivorans GRP21, exhibiting similarities of 94.7 and 92.2 %, respectively. The genomic DNA G+C content was 64.2 mol%. Based on the phenotypic, chemotaxonomic and genotypic data, strain 1-19b represents a novel species in a new genus of the family Rhodobacteraceae , for which the name Oceaniglobus indicus gen. nov., sp. nov. is proposed, with the type strain 1-19b (=MCCC 1A11863=KCTC 52709).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002275
2017-10-18
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/12/4930.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002275&mimeType=html&fmt=ahah

References

  1. Lai PY, Miao L, Lee OO, Liu LL, Zhou XJ et al. Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red sea, Saudi Arabia. Int J Syst Evol Microbiol 2013;63:1007–1012 [CrossRef][PubMed]
    [Google Scholar]
  2. Imhoff JF, Truper HG, Pfennig N. Rearrangement of the species and genera of the phototrophic "purple nonsulfur bacteria". Int J Syst Bacteriol 1984;34:340–343 [CrossRef]
    [Google Scholar]
  3. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  4. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013;29:1072–1075 [CrossRef][PubMed]
    [Google Scholar]
  5. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  6. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992;35:367–375 [CrossRef][PubMed]
    [Google Scholar]
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  9. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  11. Ostle AG, Holt JG. Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 1982;44:238–241[PubMed]
    [Google Scholar]
  12. Pukall R, Buntefuss D, Frühling A, Rohde M, Kroppenstedt RM et al. Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the alpha-Proteobacteria. Int J Syst Bacteriol 1999;49:513–519 [CrossRef][PubMed]
    [Google Scholar]
  13. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  14. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. Society for Applied Bacteriology Technical Series 1985; pp.267–287
    [Google Scholar]
  15. Kim KK, Lee JS, Lee KC, Oh HM, Kim SG. Pontibaca methylaminivorans gen. nov., sp. nov., a member of the family Rhodobacteraceae. Int J Syst Evol Microbiol 2010;60:2170–2175 [CrossRef][PubMed]
    [Google Scholar]
  16. Wu YH, Xu L, Zhou P, Wang CS, Oren A et al. Brevirhabdus pacifica gen. nov., sp. nov., isolated from deep-sea sediment in a hydrothermal vent field. Int J Syst Evol Microbiol 2015;65:3645–3651 [CrossRef][PubMed]
    [Google Scholar]
  17. Liu K, Zong R, Li Q, Fu Y, Xu Y et al. Oceaniovalibus guishaninsula gen. nov., sp. nov., a marine bacterium of the family Rhodobacteraceae. Curr Microbiol 2012;64:385–391 [CrossRef][PubMed]
    [Google Scholar]
  18. Tang K, Liu K, Jiao N. Draft genome sequence of Oceaniovalibus guishaninsula JLT2003T. J Bacteriol 2012;194:6683 [CrossRef][PubMed]
    [Google Scholar]
  19. Choi DH, Cho JC, Lanoil BD, Giovannoni SJ, Cho BC. Maribius salinus gen. nov., sp. nov., isolated from a solar saltern and Maribius pelagius sp. nov., cultured from the Sargasso sea, belonging to the Roseobacter clade. Int J Syst Evol Microbiol 2007;57:270–275 [CrossRef][PubMed]
    [Google Scholar]
  20. Albuquerque L, França L, Taborda M, La Cono V, Yakimov M et al. Palleronia abyssalis sp. nov., isolated from the deep Mediterranean Sea and the emended description of the genus Palleronia and of the species Palleronia marisminoris. Antonie van Leeuwenhoek 2015;107:633–642 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002275
Loading
/content/journal/ijsem/10.1099/ijsem.0.002275
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error