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Abstract

A novel Gram-stain-negative, facultatively anaerobic, filamentous and rose-brown pigmented bacterium, designated strain HF401, was isolated from marine sediment off the coast of Weihai, China. The isolate grew at temperatures between 4 and 45 °C (optimal growth at 33 °C), at pH 6.5–8.5 (optimal growth at pH 7.5) and with 0.5–6.0 % (w/v) NaCl (optimal growth at 3.0 %). The predominant menaquinone was menaquinone 7 (MK-7). The G+C content of the genomic DNA was 50.8 mol% (from high-performance liquid chromatography). The major fatty acids were iso-C, C anteiso-C and summed feature 3 (Cω7 and/or iso-C 2OH). The major polar lipids were phosphatidylethanolamine, phospholipid and an unidentified lipid. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HF401 formed a distinct branch with JCM 15548 in the family . The most closely related strains of strain HF401 were AP1 (96.2 % 16S rRNA gene sequence similarity), JCM 15548 (96.2 %) and SC/BZ-SP2 (96.0 %). Average nucleotide identity (ANI) values between strain HF401 and JCM 15548 showed a relatedness of 71.3 % (ANIb) and 86.0 % (ANIm). The percentage of conserved proteins (POCP) value between strain HF401 and JCM 15548 was 61.2 %. Based on polyphasic analysis, especially the phylogenetic relationships and the higher POCP value, strain HF401 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is HF401 (KCTC 42595=MCCC 1H00119).

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2017-10-01
2020-01-18
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