1887

Abstract

Three rapidly growing mycobacterial strains, QIA-37, QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752 by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7 % sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae . DNA–DNA hybridization values of 78.2 % between QIA-37 and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae . Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06 % average nucleotide identity (ANI) value with M. chelonae , slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae . Therefore, we propose an emended description of Mycobacterium chelonae , and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752(=CCUG 47445=CIP 104535=DSM 43804=JCM 6388=NCTC 946) and QIA-37 (=KCTC 39630=JCM 30986) are the type strains of the two novel subspecies.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002217
2017-09-12
2019-09-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/10/3882.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002217&mimeType=html&fmt=ahah

References

  1. Prevots DR, Marras TK. Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med 2015;36:13–34 [CrossRef][PubMed]
    [Google Scholar]
  2. Falkinham JO. Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. J Appl Microbiol 2009;107:356–367 [CrossRef][PubMed]
    [Google Scholar]
  3. Lee MR, Sheng WH, Hung CC, Yu CJ, Lee LN et al. Mycobacterium abscessus complex infections in humans. Emerg Infect Dis 2015;21:1638–1646 [CrossRef][PubMed]
    [Google Scholar]
  4. de Groote MA, Huitt G. Infections due to rapidly growing mycobacteria. Clin Infect Dis 2006;42:1756–1763 [CrossRef][PubMed]
    [Google Scholar]
  5. Brown-Elliott BA, Wallace RJ. Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria. Clin Microbiol Rev 2002;15:716–746 [CrossRef][PubMed]
    [Google Scholar]
  6. Wallace RJ, Brown BA, Onyi GO. Skin, soft tissue, and bone infections due to Mycobacterium chelonae chelonae: importance of prior corticosteroid therapy, frequency of disseminated infections, and resistance to oral antimicrobials other than clarithromycin. J Infect Dis 1992;166:405–412 [CrossRef][PubMed]
    [Google Scholar]
  7. Kusunoki S, Ezaki T. Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov. Int J Syst Bacteriol 1992;42:240–245 [CrossRef][PubMed]
    [Google Scholar]
  8. Tortoli E, Kohl TA, Brown-Elliott BA, Trovato A, Leão SC et al. Emended description of Mycobacterium abscessus, Mycobacterium abscessus subsp. abscessus and Mycobacteriumabscessus subsp. bolletii and designation of Mycobacteriumabscessus subsp. massiliense comb. nov. Int J Syst Evol Microbiol 2016;66:4471–4479 [CrossRef][PubMed]
    [Google Scholar]
  9. Magee JG, Ward AC. Genus I. Mycobacterium. In Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Suzuki K. et al (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed.vol. 5 New York: Springer; 2012; pp.312–375
    [Google Scholar]
  10. Kim BJ, Hong SH, Kook YH, Kim BJ. Mycobacterium paragordonae sp. nov., a slowly growing, scotochromogenic species closely related to Mycobacterium gordonae. Int J Syst Evol Microbiol 2014;64:39–45 [CrossRef][PubMed]
    [Google Scholar]
  11. Kim BJ, Kim JM, Kim BR, Lee SY, Kim G et al. Mycobacterium anyangense sp. nov., a rapidly growing species isolated from blood of Korean native cattle, Hanwoo (Bos taurus coreanae). Int J Syst Evol Microbiol 2015;65:2277–2285 [CrossRef][PubMed]
    [Google Scholar]
  12. Kim BJ, Math RK, Jeon CO, Yu HK, Park YG et al. Mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare. Int J Syst Evol Microbiol 2013;63:192–199 [CrossRef][PubMed]
    [Google Scholar]
  13. Lee SY, Kim BJ, Kim H, Won YS, Jeon CO et al. Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare. Int J Syst Evol Microbiol 2016;66:3132–3141 [CrossRef][PubMed]
    [Google Scholar]
  14. Kim BR, Kim JM, Kim BJ, Jang Y, Ryoo S et al. Identification of nontuberculous mycobacteria isolated from Hanwoo (Bos taurus coreanae) in South Korea by sequencing analysis targeting hsp65, rpoB and 16S rRNA genes. Vet Microbiol 2014;173:385–389 [CrossRef][PubMed]
    [Google Scholar]
  15. Kent PT, Kubica GP. Public Health Mycobacteriology: A Guide for the Level III Laboratory Centers for Disease Control and Prevention Atlanta, GA: US Department of Health and Human Services; 1985
    [Google Scholar]
  16. Pérez E, Constant P, Lemassu A, Laval F, Daffé M et al. Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J Biol Chem 2004;279:42574–42583 [CrossRef][PubMed]
    [Google Scholar]
  17. Ripoll F, Deshayes C, Pasek S, Laval F, Beretti JL et al. Genomics of glycopeptidolipid biosynthesis in Mycobacterium abscessus and M. chelonae. BMC Genomics 2007;8:114 [CrossRef][PubMed]
    [Google Scholar]
  18. Kim BJ, Yi SY, Shim TS, Do SY, Yu HK et al. Discovery of a novel hsp65 genotype within Mycobacterium massiliense associated with the rough colony morphology. PLoS One 2012;7:e38420 [CrossRef][PubMed]
    [Google Scholar]
  19. Butler WR, Thibert L, Kilburn JO. Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol 1992;30:2698–2704[PubMed]
    [Google Scholar]
  20. Choi EJ, Lee SH, Jung JY, Jeon CO. Brevibacterium jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 2013;63:3430–3436 [CrossRef][PubMed]
    [Google Scholar]
  21. Lo N, Kang HJ, Jeon CO. Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov. Int J Syst Evol Microbiol 2014;64:3768–3774 [CrossRef][PubMed]
    [Google Scholar]
  22. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  23. Kim H, Kim SH, Shim TS, Kim MN, Bai GH et al. Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65). Int J Syst Evol Microbiol 2005;55:1649–1656 [CrossRef][PubMed]
    [Google Scholar]
  24. Macheras E, Konjek J, Roux AL, Thiberge JM, Bastian S et al. Multilocus sequence typing scheme for the Mycobacterium abscessus complex. Res Microbiol 2014;165:82–90 [CrossRef][PubMed]
    [Google Scholar]
  25. Jukes TH, Cantor CR. Mammalian Protein Metabolism New York: Academic Press; 1969
    [Google Scholar]
  26. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  27. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  28. Kumar S, Nei M, Dudley J, Tamura K. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 2008;9:299–306 [CrossRef][PubMed]
    [Google Scholar]
  29. Kim BJ, Kim BR, Hong SH, Seok SH, Kook YH et al. Complete genome sequence of Mycobacterium massiliense clinical strain Asan 50594, belonging to the type II genotype. Genome Announc 2013;1:e00429-13 [CrossRef][PubMed]
    [Google Scholar]
  30. Kim BJ, Kim BR, Lee SY, Seok SH, Kook YH et al. Whole-genome sequence of a novel species, Mycobacterium yongonense DSM 45126T. Genome Announc 2013;1:e00604-13 [CrossRef][PubMed]
    [Google Scholar]
  31. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  32. Kubica GP, Baess I, Gordon RE, Jenkins PA, Kwapinski JB et al. A co-operative numerical analysis of rapidly growing mycobacteria. J Gen Microbiol 1972;73:55–70 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002217
Loading
/content/journal/ijsem/10.1099/ijsem.0.002217
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error