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Abstract

Strain 15J11-11 was isolated from soil collected at the seashore and was Gram-staining-negative, short-rod-shaped, gliding and pale-pink pigmented. Flexirubin-type pigments were absent. The isolate grew at a temperature range of 15 to 30 °C and a pH range of 7 to 8. Comparative 16S rRNA gene sequence studies showed that strain 15J11-11 belonged to the genus Larkinella within the phylum Bacteroidetes and was most closely related to Larkinella arboricola Z0532 (95.6 %), Larkinella bovis M2TB15 (95.4 %), and Larkinella insperata LMG 22510 (95.2 %). The genomic DNA G+C content of strain 15J11-11 was 53.2 mol%. The strain contained phosphatidylethanolamine, phosphatidylserine, an unidentified aminophospholipid and two unidentified polar lipids as the major polar lipids; menaquinone-7 as the predominant quinone and C16 : 1ω5c, iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids which supported the affiliation of strain 15J11-11 to the genus Larkinella . Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J11-11 represents a novel species of the genus Larkinella, for which the name Larkinella ripae sp. nov. is proposed. The type strain is 15J11-11 (=KCTC 42996=JCM 31657).

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2017-09-07
2019-10-17
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