1887

Abstract

A Gram-stain-negative, non-motile, strictly aerobic bacterium designated BW11-2 was isolated from marine sediment of the south-west Indian Ocean. Cells of BW11-2 were rod-shaped, endospore-forming, 0.3–0.5 µm wide, 1.8–2.0 µm long, catalase-positive and oxidase-negative. The isolate was capable of growing at 15–45 °C (optimum 30 °C), pH 5–9 (optimum 7) and with 0.5–10 % (w/v) NaCl (optimum 3 %). Based on 16S rRNA gene sequence similarities, BW11-2 was shown to belong to the family within the phylum and formed a distinct lineage, showing the highest sequence similarities to closely related genera: (93.9–94.7 %), (93.3–93.7 %), (93.5 %), s (92.9–93.1 %) and (92.6–93.0 %). BW11-2 shared the highest 16S rRNA gene sequence similarity with the species (94.7 %). The predominant fatty acids (>10 %) were anteiso-C and iso-C. The major quinone was menaquinone-7 (MK-7). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified aminolipid. The genomic DNA G+C content of strain BW11-2 was 43.3 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain BW11-2 represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. Strain BW11-2 (=CICC 24196=JCM 31924) is the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002133
2017-09-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3440.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002133&mimeType=html&fmt=ahah

References

  1. Ludwig W, Schleifer KH, Whitman WB. Taxonomic outline of the phylum Firmicutes. In Garrity GM, Boone DR, Castenholz RW. (editors) Bergey's Manual of Systematic Bacteriology, 3rd ed.vol. 3 New York: Springer; 2009; pp.9–13
    [Google Scholar]
  2. Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 2000;64:548–572 [CrossRef][PubMed]
    [Google Scholar]
  3. Yumoto I, Yamazaki K, Sawabe T, Nakano K, Kawasaki K et al. Bacillus horti sp. nov., a new gram-negative alkaliphilic Bacillus. Int J Syst Bacteriol 1998;48:565–571 [CrossRef][PubMed]
    [Google Scholar]
  4. Kuhnigk T, Borst EM, Breunig A, König H, Collins MD et al. Bacillus oleronius sp.nov., a member of the hindgut flora of the termite reticulitermes santonensis (Feytaud). Can J Microbiol 1995;41:699–706 [CrossRef][PubMed]
    [Google Scholar]
  5. Heyndrickx M, Coorevits A, Scheldeman P, Lebbe L, Schumann P et al. Emended descriptions of Bacillus sporothermodurans and Bacillus oleronius with the inclusion of dairy farm isolates of both species. Int J Syst Evol Microbiol 2012;62:307–314 [CrossRef][PubMed]
    [Google Scholar]
  6. Yh W, Cao Y, Wang CS, Wu M, Aharon O et al. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge. Acta Oceanol Sin 2014;33:94–104
    [Google Scholar]
  7. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  8. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  9. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  13. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014;12:635–645 [CrossRef][PubMed]
    [Google Scholar]
  14. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp.607–654
    [Google Scholar]
  15. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009;59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  16. Bernardet JF, Nakagawa Y, Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  17. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  18. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990;66:199–202 [CrossRef]
    [Google Scholar]
  19. Xie CH, Yokota A. Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 2003;49:345–349 [CrossRef][PubMed]
    [Google Scholar]
  20. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  21. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  22. Albuquerque L, Tiago I, Taborda M, Nobre MF, Veríssimo A et al. Bacillus isabeliae sp. nov., a halophilic bacterium isolated from a sea salt evaporation pond. Int J Syst Evol Microbiol 2008;58:226–230 [CrossRef][PubMed]
    [Google Scholar]
  23. Seiler H, Schmidt V, Wenning M, Scherer S. Bacillus kochii sp. nov., isolated from foods and a pharmaceuticals manufacturing site. Int J Syst Evol Microbiol 2012;62:1092–1097 [CrossRef][PubMed]
    [Google Scholar]
  24. Kosowski K, Schmidt M, Pukall R, Hause G, Kämpfer P et al. Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. Int J Syst Evol Microbiol 2014;64:88–94 [CrossRef][PubMed]
    [Google Scholar]
  25. An SY, Ishikawa S, Kasai H, Goto K, Yokota A. Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan. Int J Syst Evol Microbiol 2007;57:2489–2492 [CrossRef][PubMed]
    [Google Scholar]
  26. Hirota K, Hanaoka Y, Nodasaka Y, Yumoto I. Gracilibacillus alcaliphilus sp. nov., a facultative alkaliphile isolated from indigo fermentation liquor for dyeing. Int J Syst Evol Microbiol 2014;64:3174–3180 [CrossRef][PubMed]
    [Google Scholar]
  27. Chen YG, Cui XL, Wang YX, Zhang YQ, Tang SK et al. Virgibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 2009;59:2058–2063 [CrossRef][PubMed]
    [Google Scholar]
  28. Zavarzina DG, Tourova TP, Kolganova TV, Boulygina ES, Zhilina TN. Description of Anaerobacillus alkalilacustre gen. nov., sp. nov.—Strictly anaerobic diazotrophic Bacillus isolated from soda lake and transfer of Bacillus arseniciselenatis, Bacillus macyae, and Bacillus alkalidiazotrophicus to Anaerobacillus as the new combinations A. arseniciselenatis comb. nov., A. macyae comb. nov., and A. alkalidiazotrophicus comb. nov. Microbiology 2009;78:723–731 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002133
Loading
/content/journal/ijsem/10.1099/ijsem.0.002133
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error