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Abstract

A thermophilic poly(L-lactide)-degrading Gram-stain-positive filamentous bacterial strain that develops single spores on the aerial mycelium was isolated from forest soil at Srinagarind Dam, Kanchanaburi Province, Thailand. The results of a polyphasic taxonomic study showed that our isolate had characteristics typical of members of the genus Polycladomyces . The isolate grew aerobically at an optimum temperature of 50–55 °C and optimal pH 6–7. Meso-diaminopimelic acid was present as the diagnostic diamino acid in the peptidoglycan but no characteristic sugars are detected. The predominant menaquinone was MK–7. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine diphosphatidylglycerol, phosphatidylglycerol and phosphatidylserine. The predominant cellular fatty acid was iso–C15 : 0. The DNA G+C content of strain KSR 13 was 53.4 mol%. The 16S rRNA gene sequence analysis also indicated that strain KSR 13 belonged to the genus Polycladomyces , being most closely related to Polycladomyces abyssicola JIR-001 (99.2 %). The DNA–DNA relatedness values that distinguished KSR 13 from P. abyssicola JIR-001 were 17.8–32.1 %, which were significantly below the 70 % cutoff value recommended for species delineation. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, the new isolate is proposed as a novel species and named Polycladomyces subterraneus sp. nov. The type strain is KSR 13 (=BCC 50740=NBRC 109332).

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2017-08-25
2019-10-23
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