1887

Abstract

The characterization of five Iranian isolates, four from hospital haemodialysis water and one from the sputum of a patient, led to the detection of a novel mycobacterium species. The strains were characterized by mucoid colonies developing in 3–5 days at temperatures ranging from 25 to 37 °C. The biochemical test pattern was unremarkable while the HPLC profile of mycolic acids resembled that of Mycobacterium fortuitum . The sequences of three major housekeeping genes (16S rRNA, hsp65 and rpoB) were unique and differed from those of any other mycobacterium. Mycobacterium brisbanense , which is the species that shared the highest 16S rRNA gene sequence similarity (99.03 %), was distinct, as shown by the average nucleotide identity and by the genome to genome distance values (91.05 and 43.10 %, respectively). The strains are thus considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium aquaticum sp. nov. is proposed. The type strain is RW6 (=DSM 104277=CIP111198).

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2017-08-22
2019-10-23
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References

  1. Falkinham JO. Environmental sources of nontuberculous mycobacteria. Clin Chest Med 2015;36:35–41 [CrossRef][PubMed]
    [Google Scholar]
  2. Donohue MJ, Mistry JH, Donohue JM, O'Connell K, King D et al. Increased frequency of nontuberculous mycobacteria detection at potable water taps within the United States. Environ Sci Technol 2015;49:6127–6133 [CrossRef][PubMed]
    [Google Scholar]
  3. Falkinham JO, Hilborn ED, Arduino MJ, Pruden A, Edwards MA. Epidemiology and ecology of opportunistic premise plumbing pathogens: Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa. Environ Health Perspect 2015;123:749–758 [CrossRef][PubMed]
    [Google Scholar]
  4. Konjek J, Souded S, Guerardel Y, Trivelli X, Bernut A et al. Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system. Int J Syst Evol Microbiol 2016;66:3694–3702 [CrossRef][PubMed]
    [Google Scholar]
  5. Heidarieh P, Hashemi Shahraki A, Yaghoubfar R, Hajehasani A, Mirsaeidi M. Microbiological analysis of Hemodialysis Water in a developing country. Asaio J 2016;62:332–339 [CrossRef][PubMed]
    [Google Scholar]
  6. Kent PT, Kubica GP. Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta: U.S. Department of Health and Human Services; 1985
    [Google Scholar]
  7. CLSI Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard - Second Edition Wayne, PA: CLSI; 2011; pp.M24-A2
    [Google Scholar]
  8. CDC Standardized Method for HPLC Identification of Mycobacteria Atlanta: U.S. Department of Health and Human Services, Public Health Service; 1996
    [Google Scholar]
  9. Mcnabb A, Eisler D, Adie K, Amos M, Rodrigues M et al. Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. J Clin Microbiol 2004;42:3000–3011 [CrossRef][PubMed]
    [Google Scholar]
  10. Telenti A, Marchesi F, Balz M, Bally F, Böttger EC et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175–178[PubMed]
    [Google Scholar]
  11. Adékambi T, Colson P, Drancourt M. rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 2003;41:5699–5708 [CrossRef][PubMed]
    [Google Scholar]
  12. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014;64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  13. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  14. Lee I, Kim YO, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2015;66:1100–1103 [CrossRef][PubMed]
    [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
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