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Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped or ovoid bacterium, designated strain KEM-10, was isolated from water in an estuary environment in the Yellow Sea, South Korea, and subjected to a polyphasic taxonomic study. Strain KEM-10 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KEM-10 belonged to the genus , joining the type strain of showing 97.4 % sequence similarity. Strain KEM-10 exhibited 16S rRNA gene sequence similarity of 92.2–96.1 % to the type strains of the other species. Strain KEM-10 contained MK-7 as the predominant menaquinone and iso-C and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids detected in strain KEM-10 were phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain KEM-10 was 40.6 mol%. The mean DNA–DNA relatedness value between strain KEM-10 and the type strain of was 12 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain KEM-10 is separated from recognized species of the genus . On the basis of the data presented, strain KEM-10 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KEM-10 (=KCTC 52839=NBRC 112780).

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2017-09-01
2020-01-25
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