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Abstract

A Gram-stain-negative, motile, non-spore-forming, ovoid or rod-shaped bacterium, designated strain B15, was isolated from the culture broth of a marine microalga, sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B15 forms a stable cluster with KCTC 22669 (95.4 % 16S rRNA gene sequence similarity), CB-27A (94.9 %) and HSF11 (94.6 %) in the family Optimal growth of strain B15 was observed at 33 °C, pH 8–9 and in the presence of 3 % (w/v) NaCl. The only detected ubiquinone of strain B15 was Q-10, and the G+C content of the genomic DNA was 66.3 mol%. The major fatty acid profile comprised C cyclo 8, Cω7 ω6 and C iso I/anteiso B. The major polar lipids of strain B15 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unidentified aminolipids, and one unidentified lipid. Comprehensive analyses based on polyphasic characterization of strain B15 indicated that it represents a novel species of a new genusin the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is B15 (=KCTC 52552=MCCC 1K03221).

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2017-09-01
2020-01-25
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