1887

Abstract

A strictly anaerobic Gram-stain-positive, non-motile and non-spore-forming bacterial strain, BR31, was isolated from a faecal sample of a healthy human. Bacterial colonies were ivory-coloured on GAM agar and composed of rod-shaped cells with rounded ends approximately 1.4–2.1×0.5–0.6 µm in size. According to comparative analysis based on 16S rRNA gene sequences, strain BR31 formed a distinct phylogenetic lineage that reflects a new genus within cluster XIVa in the family , with highest similarity to DSM 3982 (94.6 %). The DNA G+C content was calculated to be 47.0 mol% from whole genome sequencing. Predicted genes associated with synthesis of teichuronic acid, polyamines, polar lipids and diaminopimelic acid were detected. The peptidoglycan contained -diaminopimelic acid and the main polar lipid detected was phosphatidylglycerol. The major metabolic end product of glucose was acetic acid, which was in agreement with those of most members of the family. However, the profile of major cellular fatty acids (C, C, summed feature 4 and C) and overall enzyme activity demonstrated phenotypic differentiation of strain BR31 from other closely related genera. Thus, based on distinct phenotypic, phylogenetic, genotypic and chemotaxonomic characteristics, strain BR31 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is BR31 (=KCTC 15482=JCM 30748).

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2017-07-01
2021-08-01
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