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Abstract

A Gram-stain-negative, facultatively anaerobic coccobacilli approximately 0.4–0.5×0.5–1.1 µm, non-motile, orange-pigmented bacterial strain, designated am2, was isolated from the coastal area of Weihai, PR China (121° 57′ E, 37° 29′ N). The temperature, pH and NaCl ranges for growth were 4–40 °C, pH 6.5–8.5 and 0.0–6.0 % (w/v) NaCl. The results of phylogenetic analyses based on 16S rRNA gene sequences revealed that am2 was phylogenetically related to members of the genus Algoriphagus and was closely related to Algoriphagus boritolerans , Algoriphagus namhaensis and Algoriphagus antarcticus with 96.4, 96.2 and 95.8 % sequence similarities, respectively. The quinone system contained menaquinone MK-7 as the predominant component. The dominant fatty acids were iso-C17 : 1ω9c,iso-C16 : 1H and iso-C15 : 0. The major polar lipids consisted of one phospholipid, phosphatidylethanolamine and six unknown lipids. The DNA G+C content was 40.6 mol%. On the basis of morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, am2 should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus marinus sp. nov. is proposed. The type strain is am2 (=KCTC 52549=MCCC 1H00178).

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2017-07-12
2019-10-15
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References

  1. Bowman JP, Nichols CM, Gibson JA. Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 2003; 53: 1343– 1355 [CrossRef] [PubMed]
    [Google Scholar]
  2. Oh KH, Kang SJ, Lee SY, Park S, Oh TK et al. Algoriphagus namhaensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62: 575– 579 [CrossRef] [PubMed]
    [Google Scholar]
  3. Lee DH, Kahng HY, Lee SB. Algoriphagus jejuensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2012; 62: 409– 413 [CrossRef] [PubMed]
    [Google Scholar]
  4. Alegado RA, Grabenstatter JD, Zuzow R, Morris A, Huang SY et al. Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate. Int J Syst Evol Microbiol 2013; 63: 163– 168 [CrossRef] [PubMed]
    [Google Scholar]
  5. Yang C, Li Y, Guo Q, Lai Q, Zheng T et al. Algoriphagus zhangzhouensis sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 2013; 63: 1621– 1626 [CrossRef] [PubMed]
    [Google Scholar]
  6. Park S, Park JM, Lee KC, Yoon JH. Algoriphagus boseongensis sp. nov., a member of the family Cyclobacteriaceae isolated from a tidal flat. Antonie van Leeuwenhoek 2014; 105: 523– 531 [CrossRef] [PubMed]
    [Google Scholar]
  7. Kang H, Weerawongwiwat V, Jung MY, Myung SC, Kim W et al. Algoriphagus chungangensis sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2013; 63: 648– 653 [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim H, Joung Y, Joh K. Algoriphagus taeanensis sp. nov., isolated from a tidal flat, and emended description of Algoriphagus hitonicola. Int J Syst Evol Microbiol 2014; 64: 21– 26 [CrossRef] [PubMed]
    [Google Scholar]
  9. Rau JE, Blotevogel KH, Fischer U. Algoriphagus aquaeductus sp. nov., isolated from a freshwater pipe. Int J Syst Evol Microbiol 2012; 62: 675– 682 [CrossRef] [PubMed]
    [Google Scholar]
  10. Inan K, Kacagan M, Ozer A, Osman Belduz A, Canakci S et al. Algoriphagus trabzonensis sp. nov., isolated from freshwater, and emended description of Algoriphagus alkaliphilus. Int J Syst Evol Microbiol 2015; 65: 2234– 2240 [CrossRef] [PubMed]
    [Google Scholar]
  11. Liu QQ, Wang Y, Li J, du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64: 2204– 2209 [CrossRef] [PubMed]
    [Google Scholar]
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30: 2725– 2729 [CrossRef] [PubMed]
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39: 783 [CrossRef]
    [Google Scholar]
  14. Bernardet JF, Nakagawa Y, Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52: 1049– 1070 [CrossRef] [PubMed]
    [Google Scholar]
  15. Dong XZ, Cai MY. (editors) Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press (in Chinese); 2001; pp. 370– 398
    [Google Scholar]
  16. CLSI Performance Standards for Antimicrobial Susceptibility Testing, Twenty-Second Informational Supplement. 2012
    [Google Scholar]
  17. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp. 330– 393
    [Google Scholar]
  18. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5: 2359– 2367 [CrossRef]
    [Google Scholar]
  19. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  20. Nedashkovskaya OI, Vancanneyt M, van Trappen S, Vandemeulebroecke K, Lysenko AM et al. Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 2004; 54: 1757– 1764 [CrossRef] [PubMed]
    [Google Scholar]
  21. Nedashkovskaya OI, Kim SB, Kwon KK, Shin DS, Luo X et al. Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 2007; 57: 1988– 1994 [CrossRef] [PubMed]
    [Google Scholar]
  22. Montero-Calasanz MC, Göker M, Rohde M, Spröer C, Schumann P et al. Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 2013; 63: 4386– 4395 [CrossRef] [PubMed]
    [Google Scholar]
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