1887

Abstract

We isolated a Gram-stain-negative, aerobic bacterial strain, 912, from a soil sample taken from Rambla Salada (Murcia), south-eastern Spain, by using the dilution-to-extinction method. Cells of the strain were motile with a polar flagellum, short rod-shaped, catalase- and oxidase-positive and grew at NaCl concentrations within the range 0–5 % (w/v) (optimum 3 %, w/v), at 4–32 °C (optimum 30 °C) and at pH 6–9 (optimum pH 7); bacteriochlorophyll a was produced. Analysis of the 16S rRNA gene sequence indicated that this strain belonged to the genus Blastomonas in the class Alphaproteobacteria . Its closest relatives were Blastomonas natatoria EY 4220, Blastomonas ursincola KR-99 and Blastomonas aquatica PE 4-5, to which the strain showed 16S rRNA gene sequence similarity values of 95.9, 95.8 and 95.1 %, respectively. The DNA G+C content was 63 mol%. The major fatty acids of strain 912 were C18 : 1ω7c/C18  : 1ω6c, C16 : 1ω7c/C16  : 1ω6c, C16 : 0 and C17 : 1ω6c. The predominant isoprenoid quinone was ubiquinone 10 (Q-10). The polar lipids contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphoglycolipid, one unidentified phospholipid and two unidentified polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus Blastomonas , for which the name Blastomonas quesadae sp. nov. is proposed. Strain 912 (=CECT 9186=LMG 29921) is the type strain.

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2017-06-20
2019-12-09
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