1887

Abstract

Strain MH, a strictly anaerobic, Gram-stain-negative, non-spore-forming, spherical coccus or coccoid-shaped microorganism, was isolated from a cow rumen during a screen for hexanoic acid-producing bacteria. The microorganism grew at 30–40 °C and pH 5.5–7.5 and exhibited production of various short- and medium-chain carboxylic acids (acetic acid, butyric acid, pentanoic acid, isobutyric acid, isovaleric acid, hexanoic acid, heptanoic acid and octanoic acid), as well as H2 and CO2 as biogas. Phylogenetic analysis based on 16S rRNA gene sequencing demonstrated that MH represents a member of the genus Megasphaera , with the closest relatives being Megapsphaera indica NMBHI-10 (94.1 % 16S rRNA sequence similarity), Megasphaera elsdenii DSM 20460 (93.8 %) and Megasphaera paucivorans DSM 16981 (93.8 %). The major cellular fatty acids produced by MH included C12 : 0, C16 : 0, C18 : 1 cis 9, and C18 : 0, and the DNA G+C content of the MH genome is 51.8 mol%. Together, the distinctive phenotypic and phylogenetic characteristics of MH indicate that this microorganism represents a novel species of the genus Megasphaera , for which the name Megasphaera hexanoica sp. nov. is herein proposed. The type strain of this species is MH (=KCCM 43214=JCM 31403).

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2017-07-26
2019-12-08
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