1887

Abstract

Strain MH, a strictly anaerobic, Gram-stain-negative, non-spore-forming, spherical coccus or coccoid-shaped microorganism, was isolated from a cow rumen during a screen for hexanoic acid-producing bacteria. The microorganism grew at 30–40 °C and pH 5.5–7.5 and exhibited production of various short- and medium-chain carboxylic acids (acetic acid, butyric acid, pentanoic acid, isobutyric acid, isovaleric acid, hexanoic acid, heptanoic acid and octanoic acid), as well as H and CO as biogas. Phylogenetic analysis based on 16S rRNA gene sequencing demonstrated that MH represents a member of the genus , with the closest relatives being NMBHI-10 (94.1 % 16S rRNA sequence similarity), DSM 20460 (93.8 %) and DSM 16981 (93.8 %). The major cellular fatty acids produced by MH included C, C, C 9, and C, and the DNA G+C content of the MH genome is 51.8 mol%. Together, the distinctive phenotypic and phylogenetic characteristics of MH indicate that this microorganism represents a novel species of the genus , for which the name sp. nov. is herein proposed. The type strain of this species is MH (=KCCM 43214=JCM 31403).

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2017-07-01
2020-09-28
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