1887

Abstract

An endophytic, short rod-shaped, non-motile and non-spore-forming actinobacterium, designated strain CPCC 204135, was isolated from a surface-sterilized medicinal plant, (Thunb.), collected from Sichuan Province, south-west China. Strain CPCC 204135 was observed to grow at temperatures between 20 and 37 °C (optimum, 28–32 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 0–9.0 % (w/v) NaCl (optimum, 0–3 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204135 belonged to the genus , showing the highest level of 16S rRNA gene sequence similarity with DSM 24323 (97.2 %), the only species of the genus in the family with a validly published name. The DNA–DNA hybridization value between strain CPCC 204135 and DSM 24323 was 20.1±1.8 %, which is far below the accepted 70 % threshold for species delineation. The cell-wall hydrolysates contained -diaminopimelic acid, alanine, glycine and glutamic acid, with the peptidoglycan type of A3γ. Diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid, one unidentified aminolipid, one unidentified polar lipid and several kinds of glycolipids were detected in the polar lipids profile. MK-9(H) was identified as the predominant menaquinone. The major cellular fatty acids (>10 %) were anteiso-C and iso-C. The G+C content of the genomic DNA of strain CPCC 204135 was determined to be 71.8 mol%. On the basis of phylogenetic analysis, and phenotypic and chemotaxonomic characteristics, we concluded that strain CPCC 204135 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain CPCC 204135 (=DSM 101717=NBRC 111773) as the type strain.

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2017-06-01
2019-12-14
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