1887

Abstract

Eight strains characterised as Gram-stain-positive, non-spore-forming and non-motile rods were isolated from samples collected from stone chambers of the Takamatsuzuka and Kitora tumuli in Asuka village, Nara Prefecture, Japan. Among them, one strain, T7528-3-6b, was shown to form a novel lineage within the genus The most closely phylogenetically related species to T7528-3-6b was , with 97.8 % sequence similarity. The major isoprenoid quinones of T7528-3-6b were MK-12, MK-13 and MK-11. The predominant cellular fatty acids for this isolate were anteiso-C, anteiso-C, iso-C and iso-C. The diagnostic diamino acid of the peptidoglycan of this isolate was ornithine. Major polar lipids of the isolate were phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The G+C content of the genomic DNA of this isolate was 70.1 mol%. On the basis of the results of physiological, biochemical and chemotaxonomic tests and molecular phylogenetic analysis, T7528-3-6b is considered to represent a novel species of the genus for which the name sp. nov. has been proposed. The type strain is T7528-3-6b (=JCM 28836=NCIMB 15039). The results of comparisons of both phenotypic and genotypic (16S rRNA gene sequence) characteristics indicated that the remaining seven isolates were very closely related to . Although the sequence similarity between the two was 99.2 %, further detailed multifaceted comparisons are needed to determine their accurate taxonomic assignment.

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2017-06-01
2020-01-17
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