1887

Abstract

Yeast surveys associated with different bromeliads in north-eastern Brazil led to the proposal of two novel yeast species, Carlosrosaea hohenbergiae sp. nov. and Carlosrosaea aechmeae sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences of the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested an affinity with a phylogenetic lineage that includes recently reclassified Carlosrosaea vrieseae. Six isolates of the novel species were obtained from different bromeliad species collected in three Atlantic Forest fragments in Alagoas state, Brazil. Ca. hohenbergiae sp. nov. differs by 69 and 12 nucleotide substitutions in the ITS and D1/D2 domain, respectively, from Ca. vrieseae. The type strain is UFMG-CM-Y405 (=BSB 34=CBS 14563), Mycobank 819227. Ca. aechmeae sp. nov. is represented by one strain isolated from Aechmea constantinii leaves. Ca. aechmeae sp. nov. differs from the related species Ca. hohenbergiae and Ca. vrieseae by 36 and 65 nucleotide substitutions, respectively, in the ITS region and by 12 and 15 nucleotide substitutions in the D1/D2 domain, respectively. The type strain of Ca. aechmeae sp. nov. is UFMG-CM-Y6095 (=BM 94=CBS 14578), Mycobank 819228.

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2017-06-14
2019-10-22
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References

  1. Zotz G. The systematic distribution of vascular epiphytes - a critical update. Bot J Linn Soc 2013;171:453–481 [CrossRef]
    [Google Scholar]
  2. Givnish TJ, Barfuss MH, van Ee B, Riina R, Schulte K et al. Adaptive radiation, correlated and contingent evolution, and net species diversification in Bromeliaceae. Mol Phylogenet Evol 2014;71:55–78 [CrossRef][PubMed]
    [Google Scholar]
  3. Basílio GA, Barbosa DEF, Furtado SG, Silva FR, Menini Neto L. Community ecology of epiphytic Bromeliaceae in a remnant of Atlantic forest in Zona da Mata, Minas Gerais State, Brazil. Hoehnea 2015;42:21–31 [CrossRef]
    [Google Scholar]
  4. Araújo FV, Rosa CA, Freitas LF, Lachance MA, Vaughan-Martini A et al. Kazachstania bromeliacearum sp. nov., a yeast species from water tanks of bromeliads. Int J Syst Evol Microbiol 2012;62:1002–1006 [CrossRef][PubMed]
    [Google Scholar]
  5. Inácio J, Landell MF, Valente P, Wang PH, Wang YT et al. Farysizyma gen. nov., an anamorphic genus in the Ustilaginales to accommodate three novel epiphytic basidiomycetous yeast species from America, Europe and Asia. FEMS Yeast Res 2008;8:499–508 [CrossRef][PubMed]
    [Google Scholar]
  6. Ruivo CC, Lachance MA, Rosa CA, Bacci M, Pagnocca FC. Candida bromeliacearum sp. nov. and Candida ubatubensis sp. nov., two yeast species isolated from the water tanks of Canistropsis seidelii (Bromeliaceae). Int J Syst Evol Microbiol 2005;55:2213–2217 [CrossRef][PubMed]
    [Google Scholar]
  7. Landell MF, Inácio J, Fonseca A, Vainstein MH, Valente P. Cryptococcus bromeliarum sp. nov., an orange-coloured basidiomycetous yeast isolated from bromeliads in Brazil. Int J Syst Evol Microbiol 2009;59:910–913 [CrossRef][PubMed]
    [Google Scholar]
  8. Landell MF, Billodre R, Ramos JP, Leoncini O, Vainstein MH et al. Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 2010;60:244–248 [CrossRef][PubMed]
    [Google Scholar]
  9. Landell MF, Brandão LR, Barbosa AC, Ramos JP, Safar SV et al. Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil. Int J Syst Evol Microbiol 2014;64:1970–1977 [CrossRef][PubMed]
    [Google Scholar]
  10. Landell MF, Brandão LR, Safar SV, Gomes FC, Félix CR et al. Bullera vrieseae sp. nov., a tremellaceous yeast species isolated from bromeliads. Int J Syst Evol Microbiol 2015;65:2466–2471 [CrossRef][PubMed]
    [Google Scholar]
  11. Safar SV, Gomes FC, Marques AR, Lachance MA, Rosa CA. Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil. Int J Syst Evol Microbiol 2013;63:1165–1168 [CrossRef][PubMed]
    [Google Scholar]
  12. Sousa FM, Morais PB, Lachance MA, Rosa CA. Hagleromyces gen. nov., a yeast genus in the Saccharomycetaceae, and description of Hagleromyces aurorensis sp. nov., isolated from water tanks of bromeliads. Int J Syst Evol Microbiol 2014;64:2915–2918 [CrossRef][PubMed]
    [Google Scholar]
  13. Gomes FC, Safar SV, Marques AR, Medeiros AO, Santos AR et al. The diversity and extracellular enzymatic activities of yeasts isolated from water tanks of Vriesea minarum, an endangered bromeliad species in Brazil, and the description of Occultifur brasiliensis f.a., sp. nov. Antonie van Leeuwenhoek 2015;107:597–611 [CrossRef][PubMed]
    [Google Scholar]
  14. Gomes F, Safar S, Santos A, Lachance M, Rosa C. Kockovaella libkindii sp. nov., a yeast species isolated from water tanks of bromeliad. Int J Syst Microbiol 2016;66:5066–5069[CrossRef]
    [Google Scholar]
  15. Machado Pagani D, Brandão LR, Santos AR, Felix CR, Pais Ramos J et al. Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., two tremellaceous yeast species from Brazil. Int J Syst Evol Microbiol 2016;66:1799–1806 [CrossRef][PubMed]
    [Google Scholar]
  16. Limtong S, Koowadjanakul N, Jindamorakot S, Yongmanitchai W, Nakase T. Candida sirachaensis sp. nov. and Candida sakaeoensis sp. nov. two anamorphic yeast species from phylloplane in Thailand. Antonie van Leeuwenhoek 2012;102:221–229 [CrossRef][PubMed]
    [Google Scholar]
  17. Camacho-Hernández IL, Chávez-Velázquez JA, Uribe-Beltrán MJ, Ríos-Morgan A, Delgado-Vargas F. Antifungal activity of fruit pulp extract from Bromelia pinguin. Fitoterapia 2002;73:411–413 [CrossRef][PubMed]
    [Google Scholar]
  18. Looby CI, Eaton WD. Effects of Bromelia pinguin (Bromeliaceae) on soil ecosystem function and fungal diversity in the lowland forests of Costa Rica. BMC Ecol 2014; [CrossRef][PubMed]
    [Google Scholar]
  19. Raffauf R et al. Diterpenoid and flavonoid constituents of Bromelia pinguin L. J Org Chem 1981;46:1094–1098[CrossRef]
    [Google Scholar]
  20. Boekhout T, Fonseca Á, Sampaio JS, Bandoni RJ, Fell JW et al. Discussion of teleomorphic and anamorphic basidiomycetous yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed.vol. III Elsevier; 2011; pp.1339–1372[CrossRef]
    [Google Scholar]
  21. Fonseca Á, Boekhout T, Fell JW. Cryptococcus Vuillemin (1901). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed.vol. III Elsevier; 2011; pp.1661–1737[CrossRef]
    [Google Scholar]
  22. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015a;81:85–147 [CrossRef][PubMed]
    [Google Scholar]
  23. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY et al. Phylogeny of Tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015b;81:1–26 [CrossRef][PubMed]
    [Google Scholar]
  24. Kurtzman CP, Fell FW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, a Taxonomic Study, 5th ed.vol. I Elsevier; 2011; pp.87–110[CrossRef]
    [Google Scholar]
  25. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications San Diego, USA: Academic Press; 1990; pp.315–322
    [Google Scholar]
  26. O’Donnell K. Fusarium and its near relatives. In Reynolds DR, Taylor JW. (editors) The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics Wallingford, UK: CAB International; 1993; pp.225–233
    [Google Scholar]
  27. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  28. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 2000;50:1351–1371 [CrossRef][PubMed]
    [Google Scholar]
  29. Lachance MA, Bowles JM, Starmer WT, Barker JS. Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 1999;45:172–177[PubMed][CrossRef]
    [Google Scholar]
  30. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  31. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  32. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  33. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef]
    [Google Scholar]
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