1887

Abstract

A Gram-stain-negative, facultatively anaerobic bacterium, designated B6, was isolated from activated sludge of a wastewater treatment plant in South Korea. Cells were oxidase- and catalase-positive and non-motile rods producing yellow carotenoid-type pigments. Growth of B6 was observed at 20–40 °C (optimum, 37 °C) and pH 6.6–8.2 (optimum, pH 7.0) and in R2A broth supplemented with 0–1 % (w/v) NaCl (optimum, 0 %). B6 contained iso-C15 : 0 as the major fatty acid. Menaquinone-6 was detected as the sole respiratory quinone. The G+C content of the genomic DNA of B6 was 31.5 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that B6 formed a tight phylogenetic lineage with members of the genus Cloacibacterium . B6 was most closely related to Cloacibacterium rupense R2A-16 (99.0 %), Cloacibacterium normanense NRS1 (98.7 %) and Cloacibacterium haliotis WB5 (97.4 %), but their DNA–DNA relatedness levels were less than 42.0 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that B6 represents a novel species of the genus Cloacibacterium , for which the name Cloacibacterium caeni sp. nov. is proposed. The type strain is B6 (=KACC 18988=JCM 31714).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001841
2017-06-07
2019-08-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/6/1688.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001841&mimeType=html&fmt=ahah

References

  1. Allen TD, Lawson PA, Collins MD, Falsen E, Tanner RS. Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family Flavobacteriaceae isolated from municipal wastewater. Int J Syst Evol Microbiol 2006;56:1311–1316 [CrossRef][PubMed]
    [Google Scholar]
  2. Cao SJ, Deng CP, Li BZ, Dong XQ, Yuan HL. Cloacibacterium rupense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 2010;60:2023–2026 [CrossRef][PubMed]
    [Google Scholar]
  3. Hyun DW, Shin NR, Kim MS, Kim JY, Kim PS et al. Cloacibacterium haliotis sp. nov., isolated from the gut of an abalone, Haliotis discus hannai. Int J Syst Evol Microbiol 2014;64:72–77 [CrossRef][PubMed]
    [Google Scholar]
  4. Jeong SH, Jin HM, Lee HJ, Jeon CO. Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 2013;63:971–976 [CrossRef][PubMed]
    [Google Scholar]
  5. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  6. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007;3:e56 [CrossRef][PubMed]
    [Google Scholar]
  7. Felsenstein J. PHYLIP (phylogeny inference package), Version 3.6a. Department of Genetics, University of Washington, Seattle, WA, USA: 2002
    [Google Scholar]
  8. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014;30:1312–1313 [CrossRef][PubMed]
    [Google Scholar]
  9. Chang HW, Nam YD, Jung MY, Kim KH, Roh SW et al. Statistical superiority of genome-probing microarrays as genomic DNA–DNA hybridization in revealing the bacterial phylogenetic relationship compared to conventional methods. J Microbiol Methods 2008;75:523–530 [CrossRef][PubMed]
    [Google Scholar]
  10. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002;52:1043–1047 [CrossRef][PubMed]
    [Google Scholar]
  11. Bernardet JF, Nakagawa Y, Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  12. Schmidt K, Connor A, Britton G. Analysis of pigments: carotenoids and related polyenes. In Goodfellow M, O’Donnell AG. (editors) Chemical Methods in Prokaryotic Systematics New York: Wiley; 1994; pp.403–461
    [Google Scholar]
  13. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002;4:770–773 [CrossRef][PubMed]
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  15. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–208[CrossRef]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001841
Loading
/content/journal/ijsem/10.1099/ijsem.0.001841
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error