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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 5

gen. nov., sp. nov., a moderately halophilic member of the family Free

Abstract

A novel Gram-stain-negative, rod-shaped, facultatively anaerobic, oxidase-negative and catalase-positive bacterium, designated JZ3C29, was isolated from a saltern located in Feicheng, PR China. JZ3C29 was tolerant of moderately saline conditions. Optimal growth occurred at 40 °C (range 20–50 °C) and pH 7.5–8.5 (range pH 7.0–9.0) with 8–10 % (w/v) NaCl (range 2–16 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that JZ3C29 shared highest similarity with CL-CB462 (90.5 %), YIM J14 (90.5 %) and CL-KR2 (90.4 %) and less than 90.0 % similarity with other species of the phylum . The isolate formed a novel genus-level clade in the recently described family . The polar lipid profile of the novel isolate consisted of diphosphatidylglycerol, phosphatidylethanolamine, three unidentified glycolipids, four unidentified phospholipids and two unidentified lipids. The dominant cellular fatty acids (>10 %) were summed feature 3 (Cω7 and/or iso-C 2-OH) and iso-C and the sole respiratory quinone was menaquinone 7 (MK-7). The DNA G+C content of JZ3C29 was 44.4 mol%. On the basis of these phenotypic and phylogenetic data, JZ3C29 should be classified as representing a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is JZ3C29 (=MCCC 1H00131=KCTC 52046=JCM 31413).

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Keyword(s): 16S rRNA , phylogenetic analysis and Rhodohalobacter halophilus gen. nov., sp. nov.
© 2017 IUMS
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2017-05-01
2025-01-16
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Rhodohalobacter halophilus gen. nov., sp. nov., a moderately halophilic member of the family Balneolaceae
Int J Syst Evol Microbiol 67, 1281 (2017); https://doi.org/10.1099/ijsem.0.001806
/content/journal/ijsem/10.1099/ijsem.0.001806
/content/journal/ijsem/10.1099/ijsem.0.001806
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