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Volume 67, Issue 5

sp. nov., isolated from surface sediment and emended description of the genus Free

Abstract

A bacterial strain, designated JS7-11, was isolated from the surface sediment of the Jiulong River and characterized using a polyphasic taxonomic approach. Cells of JS7-11 were Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 10–40 °C (optimum 25–30 °C), at pH 6–8 (optimum 7) and in 1–12 % NaCl (optimum 3–4 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that JS7-11 and SSW136 formed an independent cluster, sharing 97.9 % similarity between the two strains, and less than 96.0 % similarities with other type strains of members of the family . The major fatty acids were summed feature 8 and C. The quinone was identified as Q-10. The polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 63.4 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between JS7-11 and SSW136 were 22.1 and 79.4 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses clearly indicated that JS7-11 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain of the novel species is JS7-11 (=MCCC 1A00710=KCTC 42144).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): dDDH , nioella sediminis , taxonomy and the surface sediment
© 2017 IUMS
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2017-05-01
2024-12-07
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References

  1. Du J, Dong C, Lai Q, Liu Y, Xie Y et al. Pseudobowmanella zhangzhouensis gen. nov., sp. nov., isolated from the surface freshwater of the Jiulong river in China. Antonie van Leeuwenhoek 2015; 107:741–748 [View Article][PubMed]
     [Google Scholar]
  2. Du J, Liu Y, Lai Q, Dong C, Xie Y et al. Kordia zhangzhouensis sp. nov., isolated from surface freshwater. Int J Syst Evol Microbiol 2015; 65:3379–3383 [View Article][PubMed]
     [Google Scholar]
  3. Liu Y, Du J, Lai Q, Dong C, Xie Y et al. Jiulongibacter sediminis gen. nov., sp. nov., a new member of the family Cytophagaceae, isolated from the surface sediment of the Jiulong river in China. Int J Syst Evol Microbiol 2016; 66:2347–2353 [View Article][PubMed]
     [Google Scholar]
  4. Rajasabapathy R, Mohandass C, Yoon JH, Dastager SG, Liu Q et al. Nioella nitratireducens gen. nov., sp. nov., a novel member of the family Rhodobacteraceae isolated from Azorean Island. Antonie van Leeuwenhoek 2015; 107:589–595 [View Article][PubMed]
     [Google Scholar]
  5. Pujalte MJ, Lucena T, Ruvira MA, Arahal DR, Macián MC et al. The family Rhodobacteraceae. In: The Prokaryotes: Alphaproteobacteria and Betaproteobacteria 2014 pp. 439–512 [CrossRef]
     [Google Scholar]
  6. Collee JG, Miles R, Watt B. Tests for identification of bacteria. In Collee JG, Fraser AG, Marmion BP, Simmons A. (editors) Mackie & McCartney. Practical Medical Microbiology, 14th ed. New York: Churchill Livingstone; 1996 pp. 131–149
     [Google Scholar]
  7. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation); 2001
     [Google Scholar]
  8. Lai Q, Liu Y, Shao Z. Bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (Mugil cephalus). Antonie van Leeuwenhoek 2014; 105:99–107 [View Article][PubMed]
     [Google Scholar]
  9. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  10. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
     [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  13. Swofford DL, Berlocher SH. Inferring evolutionary trees from gene frequency data under the principle of maximum parsimony. Syst Biol 1987; 36:293–325
     [Google Scholar]
  14. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article]
     [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
     [Google Scholar]
  16. Collins M. Isoprenoid quinone analyses in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series No. 20) London: Academic Press; 1985 pp. 267–287
     [Google Scholar]
  17. Kates M. Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids Amsterdam: North Holland Publishing Company; 1986
     [Google Scholar]
  18. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  19. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  20. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
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Nioella sediminis sp. nov., isolated from surface sediment and emended description of the genus Nioella
Int J Syst Evol Microbiol 67, 1271 (2017); https://doi.org/10.1099/ijsem.0.001798
/content/journal/ijsem/10.1099/ijsem.0.001798
/content/journal/ijsem/10.1099/ijsem.0.001798
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