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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F3P, was isolated from the Han River, South Korea, and characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed 16F3P to be within the genus Hymenobacter , and most closely related to Hymenobacter chitinivorans Txc1 (98.62 %) and Hymenobacter elongatus VUG-A112 (98.46 %). The phylogenetic distance from other species of the genus Hymenobacter with validly published names was greater than 4 % (i.e. sequence similarity was less than 96.0 %). Chemotaxonomic data also supported the classification of strain 16F3P within the genus Hymenobacter . C16 : 0 (19.8 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 15.4 %) and iso-C15 : 0 (13.0 %) were the major fatty acids, MK-7 was the predominant respiratory quinone, and phosphatidylethanolamine was the major polar lipid. The G+C content of the genomic DNA of strain 16F3P was 61.9 mol%. DNA–DNA hybridization experiments showed that the values for DNA–DNA relatedness between strain 16F3P and the phylogenetically closest neighbours were below 19 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 16F3P represents a novel species of the genus Hymenobacter , for which the name Hymenobacter aquaticus sp. nov. is proposed. The type strain is 16F3P (=KCTC 52194=JCM 31653).

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2017-05-30
2019-10-24
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