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Abstract

A novel actinobacterium, designated KLBMP 5180, was isolated from the surface-sterilized root of a coastal halophyte, , collected from the city of Lianyungang, Jiangsu Province, eastern China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, one unidentified glycolipid and two unidentified lipids. anteiso-C and iso-C were the major cellular fatty acids. The DNA G+C content of strain KLBMP 5180 was 60.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KLBMP 5180 belongs to the genus and was related most closely to DSM 20123 (99.3 % similarity), Re117 (99.3 %) and LMG 16219 (99.1 %); similarity to other type strains of the genus was lower than 98.5 %. However, DNA–DNA relatedness values between strain KLBMP 5180, DSM 20123, Re117 and LMG 16219 were 47.5±2.6, 51.3±3.1 and 41.2±4.3 %, respectively. The combination of DNA–DNA hybridization, phylogenetic, phenotypic and chemotaxonomic data supported the suggestion that strain KLBMP 5180 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KLBMP 5180 (=DSM 101718=KCTC 39692).

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2017-05-01
2019-12-11
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