1887

Abstract

During the summers of 2013 and 2014, isolates of a novel Gram-stain-negative coccus in the genus Neisseria were obtained from the contents of nonviable greater white-fronted goose (Anseralbifrons) eggs on the Arctic Coastal Plain of Alaska. We used a polyphasic approach to determine whether these isolates represent a novel species. 16S rRNA gene sequences, 23S rRNA gene sequences, and chaperonin 60 gene sequences suggested that these Alaskan isolates are members of a distinct species that is most closely related to Neisseria canis , N eisseria animaloris and N eisseria shayeganii . Analysis of the rplF gene additionally showed that the isolates are unique and most closely related to N eisseria weaveri . Average nucleotide identity of the whole genome sequence of the type strain was between 71.5 and 74.6 % compared to close relatives, further supporting designation as a novel species. Fatty acid methyl ester analysis showed a predominance of C14 : 0, C16 : 0 and C16 : 1ω7c fatty acids. Finally, biochemical characteristics distinguished the isolates from other species of the genus Neisseria . On the basis of these combined data, the isolates are proposed to represent a novel species of the genus Neisseria , with the name Neisseria arctica sp. nov. The type strain is KH1503 (=ATCC TSD-57=DSM 103136).

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2017-06-05
2019-10-18
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