1887

Abstract

A novel cellobiose-degrading and lactate-producing bacterium, strain Cavy grass 6, was isolated from faecal samples of guinea pigs (Cavia porcellus). Cells of the strain were ovalshaped, non-motile, non-spore-forming, Gram-stain-positive and facultatively anaerobic. The strain gr at 25–40 °C (optimum 37 °C) and pH 4.5–9.5 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cavy grass 6 belongs to the genus Streptococcus with its closest relative being Streptococcus devriesei CCUG 47155 with only 96.5 % similarity. Comparing strain Cavy grass 6 and Streptococcus devriesei CCUG 47155, average nucleotide identity and level of digital DNA–DNA hybridization dDDH were only 86.9 and 33.3 %, respectively. Housekeeping genes groEL and gyrA were different between strain Cavy grass 6 and other streptococci. The G+C content of strain Cavy grass 6 was 42.6±0.3 mol%. The major (>10 %) cellular fatty acids of strain Cavy grass 6 were C16:0, C20 : 1ω9c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). Strain Cavy grass 6 ferment a range of plant mono- and disaccharides as well as polymeric carbohydrates, including cellobiose, dulcitol, d-glucose, maltose, raffinose, sucrose, l-sorbose, trehalose, inulin and dried grass extract, to lactate, formate, acetate and ethanol. Based on phylogenetic and physiological characteristics, Cavy grass 6 can be distinguished from other members of the genus Streptococcus . Therefore, a novel species of the genus Streptococcus , family Streptococcaceae , order Lactobacillales is proposed, Streptococcus caviae sp. nov. (type strain Cavy grass 6=TISTR 2371=DSM 102819).

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2017-05-25
2019-10-22
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