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Abstract

A polyphasic taxonomic approach was used to characterize a presumably novel bacterium, designated strain CC-YTH161, isolated from an agarwood sample. Cells of strain CC-YTH161 were Gram-stain-positive aerobic rods, which grew at 20–40 °C, at pH 5.0–9.0 and with 0–5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH161 appeared to belong to the genus Humibacter , and was closely related to Humibacter antri D7-27 (96.6 % similarity) and Humibacter ginsengiterrae DCY60 (96.2 %). The DNA G+C content was 67.0 mol% and the predominant quinone system was menaquinones (MK) 11 and 12. The major cellular fatty acids of the isolate were C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and C18 : 1ω7c/C18  : 1ω6c. The polar lipid profile comprised predominant amounts of diphosphatidylglycerol followed by two unidentified co-migrating glycolipids and phosphatidylglycerol in significant amounts. The diagnostic diamino acid was 2,4-diaminobutyric acid. All these features confirmed the placement of strain CC-YTH161 within the genus Humibacter . On the basis of evidence from this study, strain CC-YTH161 is considered to represent a novel species of the genus Humibacter , for which the name Humibacter aquilariae sp. nov. is proposed. The type strain is CC-YTH161 (=BCRC 80936=JCM 31199).

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2017-05-24
2019-12-09
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