1887

Abstract

Strains 97/79 and A121, recovered respectively from human faeces and well water, were compared to currently known species of the genus Citrobacter using genotypic and phenotypic approaches. Multilocus sequence analysis based on housekeeping genes fusA, leuS, pyrG, rpoB and recN, showed that the two strains formed a distinct phylogenetic lineage within the genus Citrobacter . Average nucleotide identity (ANI) between strains 97/79 and A121 was 99.2 %, whereas ANI values of strain 97/79 with the type strains of closely related species of the genus Citrobacter , C. werkmanii , C. braakii , C. freundii , C. youngae and C. pasteurii , were all below 93.0 %. The ability to metabolize different compounds also discriminated strains 97/79 and A121 from other species of the genus Citrobacter . Based on these results, strains 97/79 and A121 represent a novel species of the genus Citrobacter , for which the name Citrobacter europaeus sp. nov. is proposed, with strain 97/79 (=CIP 106467=DSM 103031) as the type strain. The DNA G+C content of strain 97/79 is 52.0 %.

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2017-02-20
2019-10-14
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References

  1. Borenshtein D, Schauer D. The genus Citrobacter. In Dworkin M, Falkow S, Rosenberg E, Scheleifer KH, Stackebrandt E et al. (editors) The Prokaryotes New York: Springer; 2006; pp.90–98[CrossRef]
    [Google Scholar]
  2. Engelkirk PG, Duben-Engelkirk PG. Laboratory diagnosis of infectious diseases: essentials of diagnostic microbiology. The Gram-Negative Bacilli: The Family Enterobacteriaceae Philadelphia: Lippincott Williams & Wilkins; 2008; pp308
    [Google Scholar]
  3. Ribeiro TG, Novais Â, Branquinho R, Machado E, Peixe L. Phylogeny and comparative Genomics unveil independent Diversification Trajectories of qnrB and genetic Platforms within particular Citrobacter Species. Antimicrob Agents Chemother 2015;59:5951–5958 [CrossRef][PubMed]
    [Google Scholar]
  4. Albaser A, Kazana E, Bennett MH, Cebeci F, Luang-In V et al. Discovery of a bacterial glycoside hydrolase family 3 (GH3) β-glucosidase with myrosinase activity from a Citrobacter strain isolated from soil. J Agric Food Chem 2016;64:1520–1527 [CrossRef][PubMed]
    [Google Scholar]
  5. Clermont D, Motreff L, Passet V, Fernandez JC, Bizet C et al. Multilocus sequence analysis of the genus Citrobacter and description of Citrobacter pasteurii sp. nov. Int J Syst Evol Microbiol 2015;65:1486–1490 [CrossRef][PubMed]
    [Google Scholar]
  6. Ko KS, Choi JY, Kim J, Park MK. Citrobacter bitternis sp. nov. isolated from bitterns. Curr Microbiol 2015;70:894–897 [CrossRef][PubMed]
    [Google Scholar]
  7. Brenner DJ, Grimont PA, Steigerwalt AG, Fanning GR, Ageron E et al. Classification of citrobacteria by DNA hybridization: designation of Citrobacter farmeri sp. nov., Citrobacter youngae sp. nov., Citrobacter braakii sp. nov., Citrobacter werkmanii sp. nov., Citrobacter sedlakii sp. nov., and three unnamed Citrobacter genomospecies. Int J Syst Bacteriol 1993;43:645–658 [CrossRef][PubMed]
    [Google Scholar]
  8. Frederiksen W. Genus X. Citrobacter Werkman and Gillen. In Brenner D, Krieg N, Staley J, Garrity G. (editor) Bergey's Manual of Systematic Bacteriology New York, USA: Springer; 2005; pp.651–656
    [Google Scholar]
  9. Delétoile A, Decré D, Courant S, Passet V, Audo J et al. Phylogeny and identification of Pantoea species and typing of Pantoea agglomerans strains by multilocus gene sequencing. J Clin Microbiol 2009;47:300–310 [CrossRef][PubMed]
    [Google Scholar]
  10. Warren JR, Farmer JJ, Dewhirst FE, Birkhead K, Zembower T et al. Outbreak of nosocomial infections due to extended-spectrum beta-lactamase-producing strains of enteric group 137, a new member of the family Enterobacteriaceae closely related to Citrobacter farmeri and Citrobacter amalonaticus. J Clin Microbiol 2000;38:3946–3952[PubMed]
    [Google Scholar]
  11. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  12. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  13. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 2003;52:696–704 [CrossRef][PubMed]
    [Google Scholar]
  14. Naum M, Brown EW, Mason-Gamer RJ. Is 16S rDNA a reliable phylogenetic marker to characterize relationships below the family level in the Enterobacteriaceae?. J Mol Evol 2008;66:630–642 [CrossRef][PubMed]
    [Google Scholar]
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