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Abstract

A Gram-stain-negative, non-motile, yellow-pigmented bacterium, designated strain IMCC27201, was isolated from an artificial freshwater pond (Inkyong) in Korea. Growth of strain IMCC27201 occurred at 10–37 °C (optimum, 30 °C), at pH 7.0–8.0 (optimum, pH 7.0) and with 0–0.25 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC27201 belonged to the genus Flavobacterium and was related most closely to Flavobacterium columnare IFO 15943 (97.8 % sequence similarity), Flavobacterium terrae R2A1-13 (97.2 %) and Flavobacterium vireti THG-SM1 (96.4 %). Strain IMCC27201 exhibited low DNA–DNA relatedness with F. columnare KCTC 42885 (34.9–40.6 %). The DNA G+C content of strain IMCC27201 was 32.1 mol%. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C15 : 0 3-OH. Strain IMCC27201 contained menaquinone-6 (MK-6) as the sole isoprenoid quinone. The polar lipids of strain IMCC27201 consisted of phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene phylogeny, phenotypic characterization and DNA–DNA relatedness, strain IMCC27201represents a novel species of the genus Flavobacterium , for which the name Flavobacterium inkyongense sp. nov. is proposed. The type strain is IMCC27201 (=KCTC 52244=JCM 31385).

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2017-02-06
2019-10-20
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