1887

Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated RA2-7, was isolated from a mussel (Mytilus edulis) collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA2-7 grew optimally at 20 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain RA2-7 belonged to the genus Colwellia . Strain RA2-7 exhibited 16S rRNA gene sequence similarity values of 98.3, 98.0 and 97.5 % to the type strains of Colwellia sediminilitoris , Colwellia aestuarii and Colwellia polaris , respectively, and of 94.5–96.5 % to the type strains of the other species of the genus Colwellia . Strain RA2-7 contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C16 : 0 as the major fatty acids. The major polar lipids detected in strain RA2-7 were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain RA2-7 was 39.0±0.04 mol% and its DNA–DNA relatedness values with the type strains of C . sediminilitoris , C. aestuarii and C. polaris were 14–19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain RA2-7 is separated from recognized species of the genus Colwellia . On the basis of the data presented, strain RA2-7 is considered to represent a novel species of the genus Colwellia , for which the name Colwellia mytili sp. nov. is proposed. The type strain is RA2-7 (=KCTC 52417=NBRC 112381).

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2017-02-20
2019-10-14
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