1887

Abstract

A Gram-negative, aerobic, short rod-shaped, non-motile, pale-yellow bacterial strain was isolated from biological soil crusts collected in the desert of Kubuqi, Inner Mongolia of China, designated MIMBbqt12. The G+C content of the genomic DNA of strain MIMBbqt12 was 49.5 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain MIMBbqt12 belongs to the genus with the highest sequence similarity of 93.5 % to R2-35. The major fatty acids of strain MIMBbqt12 were C, iso-C, iso-C3-OH, Cω5 and summed feature 3 (Cω6 and/or Cω7). The results of phenotypic properties and phylogenetic distinctiveness supported that strain MIMBbqt12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MIMBbqt12 (=MCCC 1K01308=KCTC 42805).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001558
2016-12-01
2024-11-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5568.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001558&mimeType=html&fmt=ahah

References

  1. Ahn J.-H., Weon H.-Y., Kim S.-J., Hong S.-B., Seok S.-J., Kwon S.-W. 2014; Spirosoma oryzae sp. nov., isolated from rice soil and emended description of the genus Spirosoma . Int J Syst Evol Microbiol 64:3230–3234 [View Article][PubMed]
    [Google Scholar]
  2. Baik K. S., Kim M. S., Park S. C., Lee D. W., Lee S. D., Ka J. O., Choi S. K., Seong C. N. 2007; Spirosoma rigui sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57:2870–2873 [View Article][PubMed]
    [Google Scholar]
  3. Bates S. T., Nash T. H., Sweat K. G., Garcia-Pichel F. 2010; Fungal communities of lichen-dominated biological soil crusts: diversity, relative microbial biomass, and their relationship to disturbance and crust cover. J Arid Environ 74:1192–1199 [View Article]
    [Google Scholar]
  4. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496[PubMed]
    [Google Scholar]
  5. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  6. Chang X., Jiang F., Wang T., Kan W., Qu Z., Ren L., Fang C., Peng F. 2014; Spirosoma arcticum sp. nov., isolated from high arctic glacial till. Int J Syst Evol Microbiol 64:2233–2237 [View Article][PubMed]
    [Google Scholar]
  7. Concostrina-Zubiri L., Huber-Sannwald E., Martínez I., Flores Flores J. L., Escudero A. 2013; Biological soil crusts greatly contribute to small-scale soil heterogeneity along a grazing gradient. Soil Biol Biochem 64:28–36 [View Article]
    [Google Scholar]
  8. Dong X., Cai M. 2001 Determinative manual for routine bacteriology Beijing: Scientific Press (English translation);
    [Google Scholar]
  9. Du H., Jiao N., Hu Y., Zeng Y. 2006; Diversity and distribution of pigmented heterotrophic bacteria in marine environments. FEMS Microbiol Ecol 57:92–105 [View Article][PubMed]
    [Google Scholar]
  10. Edgar R. C. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  13. Finster K. W., Herbert R. A., Lomstein B. A. 2009; Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma . Int J Syst Evol Microbiol 59:839–844 [View Article][PubMed]
    [Google Scholar]
  14. Fries J., Pfeiffer S., Kuffner M., Sessitsch A. 2013; Spirosoma endophyticum sp. nov., isolated from Zn- and Cd-accumulating Salix caprea. Int J Syst Evol Microbiol 63:4586–4590 [View Article][PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  16. Kimura M., Takahata N. 1983; Selective constraint in protein polymorphism: study of the effectively neutral mutation model by using an improved pseudosampling method. Proc Natl Acad Sci USA 80:1048–1052 [View Article]
    [Google Scholar]
  17. Kumar S., Stecher G., Tamura K. 2016; mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  18. Larkin J. M., Borrall R. 1984; Family 1. Spirosomaceae Larkin and Borrall 1978 595AL. In Bergey’s Manual of Systematic Bacteriology vol. 1 pp. 125–132 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2:233–241 [View Article]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  21. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, Del: Microbial ID Inc;
    [Google Scholar]
  22. Skerman V. 1967 A Guide to the Identification of the Genera of Bacteria Baltimore: Williams & Wilkins;
    [Google Scholar]
  23. Swindell S. R., Plasterer T. N. 1997; SEQMAN. In Sequence Data Analysis Guidebook pp. 75–89 Springer; [CrossRef]
    [Google Scholar]
  24. Ten L. N., Xu J. L., Jin F. X., Im W. T., Oh H. M., Lee S. T. 2009; Spirosoma panaciterrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:331–335 [View Article][PubMed]
    [Google Scholar]
  25. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  26. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001558
Loading
/content/journal/ijsem/10.1099/ijsem.0.001558
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error