1887

Abstract

A Gram-stain-negative, short rod-shaped and light-red-pigmented bacterium, designated XAAS-1, was isolated from the soil of Aydingkol Lake near the Turpan City, Xinjiang, China. The isolate was positive for oxidase, catalase and hydrolysis of starch, casein, gelatin and aesculin. The sole respiratory quinone was MK-7 and the principal cellular fatty acids were iso-C15 : 0 and C15 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid and two unidentified polar lipids. The polyamine pattern was found to contain mainly sym-homospermidine. 16S rRNA gene sequence analysis indicated that strain XAAS-1 belongs to the genus Pontibacter in the family Cytophagaceae , with sequence similarities ranging from 93.8 to 96.7 % with other type species of the genus Pontibacter . On the basis of phenotypic and genotypic data, strain XAAS-1 represents a novel species of the genus Pontibacter , for which the name Pontibacter aydingkolensis sp. nov. (type strain XAAS-1=CCTCC AB 2016134=JCM 31442) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001551
2016-12-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5523.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001551&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B.. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52: 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bowman J. P..( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50: 1861–1868. [CrossRef] [PubMed]
    [Google Scholar]
  3. Busse H.-J., Bunka S., Hensel A., Lubitz W..( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. . Int J Syst Bacteriol 47: 698–708. [CrossRef]
    [Google Scholar]
  4. Cao H., Nie Y., Zeng X. C., Xu L., He Z., Luo X., Wu R..( 2014;). Pontibacter yuliensis sp. nov., isolated from soil. . Int J Syst Evol Micr 64: 968–972. [CrossRef]
    [Google Scholar]
  5. Cerny G..( 1978;). Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. . Appl Microbiol Biotechnol 5: 113–122. [CrossRef]
    [Google Scholar]
  6. Collins M. D..( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London:: Academic Press;.
    [Google Scholar]
  7. Dai J., Xu M., Peng F., Jiang F., Chen X., Wang Z., Fang C..( 2014;). Pontibacter soli sp. nov., isolated from the soil of a Populus rhizosphere in Xinjiang, China. . Antonie van Leeuwenhoek 105: 65–72. [CrossRef] [PubMed]
    [Google Scholar]
  8. Dastager S. G., Raziuddin Q. S., Deepa C. K., Li W. J., Pandey A..( 2010;). Pontibacter niistensis sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60: 2867–2870. [CrossRef] [PubMed]
    [Google Scholar]
  9. Dwivedi V., Niharika N., Lal R..( 2013;). Pontibacter lucknowensis sp. nov., isolated from a hexachlorocyclohexane dump site. . Int J Syst Evol Microbiol 63: 309–313. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jordan E. M., Thompson F. L., Zhang X.-H., Li Y., Vancanneyt M., Kroppenstedt R. M., Priest F. G., Austin B..( 2007;). Sneathiella chinensis gen. nov., sp. nov., a novel marine alphaproteobacterium isolated from coastal sediment in Qingdao, China. . Int J Syst Evol Microbiol 57: 114–121. [CrossRef] [PubMed]
    [Google Scholar]
  11. Joung Y., Kim H., Ahn T. S., Jon K..( 2011;). Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea. . J Microbiol 49: 290–293. [CrossRef] [PubMed]
    [Google Scholar]
  12. Joung Y., Kim H., Lee B.-I., Kang H., Jang T. Y., Kwon O. S., Joh K..( 2013;). Pontibacter jeungdoensis sp. nov., isolated from a solar saltern in Korea. . J Microbiol 51: 531–535. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kang J. Y., Joung Y., Chun J., Kim H., Joh K., Jahng K. Y..( 2013;). Pontibacter saemangeumensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 63: 565–569. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J.-H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim D. I., Lee J. H., Kang J. W., Ka J. O., Seong C. N..( 2016;). Pontibacter rugosus sp. nov., isolated from seawater. . Int J Syst Evol Microbiol. doi: 10.1099/ijsem.0.001362. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kohli P., Nayyar N., Lal R., Sharma A., Singh A. K..( 2016;). Pontibacter virosus sp. nov., isolated from a hexachlorocyclohexane contaminated dumpsite. . Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.001363.
    [Google Scholar]
  17. Kroppenstedt R. M..( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. . J Liq Chromatogr 5: 2359–2367. [CrossRef]
    [Google Scholar]
  18. Lányí B..( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19: 1–67.[CrossRef]
    [Google Scholar]
  19. Mahato N. K., Nayyar N., Lal R., Singh A. K., Tripathi C..( 2016;). Pontibacter ummariensis sp. nov., isolated from a hexachlorocyclohexane-contaminated soil. . Int J Syst Evol Microbiol 66: 1080–1087. [CrossRef]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159–167. [CrossRef]
    [Google Scholar]
  21. Minnikin D. E., Collins M. D., Goodfellow M..( 1979;). Fatty acid and polar lipid 314 composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47: 87–95. [CrossRef]
    [Google Scholar]
  22. Nedashkovskaya O. I., Kim S. B., Suzuki M., Shevchenko L. S., Lee M. S., Lee K. H., Park M. S., Frolova G. M., Oh H. W. et al.( 2005;). Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘Bacteroidetes,’ and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. . Int J Syst Evol Microbiol 55: 2583–2588. [CrossRef] [PubMed]
    [Google Scholar]
  23. Park S., Park J. M., Lee K. H., Yoon J. H..( 2016;). Pontibacter litorisediminis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 66: 4172–4178. [CrossRef] [PubMed]
    [Google Scholar]
  24. Raichand R., Kaur I., Singh N. K., Mayilraj S..( 2011;). Pontibacter rhizosphera sp. nov., isolated from rhizosphere soil of an Indian medicinal plant Nerium indicum. . Antonie van Leeuwenhoek 100: 129–135. [CrossRef] [PubMed]
    [Google Scholar]
  25. Singh A. K., Garg N., Lata P., Kumar R., Negi V., Vikram S., Lal R..( 2014;). Pontibacter indicus sp. nov., isolated from hexachlorocyclohexane-contaminated soil. . Int J Syst Evol Microbiol 64: 254–259. [CrossRef] [PubMed]
    [Google Scholar]
  26. Singh A. K., Garg N., Lal R..( 2015;). Pontibacter chinhatensis sp. nov., isolated from pond sediment containing discarded hexachlorocyclohexane isomer waste. . Int J Syst Evol Microbiol 65: 2248–2254. [CrossRef] [PubMed]
    [Google Scholar]
  27. Smibert R. M., Krieg N. R..( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Srinivasan S., Lee J. J., Lee S. S., Kim M. K..( 2014;). Pontibacter humi sp. nov., isolated from mountain soil. . Curr Microbiol 69: 263–269. [CrossRef] [PubMed]
    [Google Scholar]
  29. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V..( 2013;). Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. . Int J Syst Evol Microbiol 63: 4524–4532. [CrossRef] [PubMed]
    [Google Scholar]
  30. Subhash Y., Sasikala Ch., Ramana Ch. V..( 2014;). Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert. . Int J Syst Evol Microbiol 64: 1006–1011. [CrossRef] [PubMed]
    [Google Scholar]
  31. Suresh K., Mayilraj S., Chakrabarti T..( 2006;). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Flexibacteraceae’. . Int J Syst Evol Microbiol 56: 1703–1707. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  33. Wu Y. H., Zhou P., Jian S. L., Liu Z. S., Wang C. S., Oren A., Xu X. W..( 2016;). Pontibacter amylolyticus sp. nov., isolated from a deep-sea sediment hydrothermal vent field. . Int J Syst Evol Microbiol 66: 1760–1767. [CrossRef] [PubMed]
    [Google Scholar]
  34. Xu M., Wang Y., Dai J., Jiang F., Rahman E., Peng F., Fang C..( 2012;). Pontibacter populi sp. nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest. . Int J Syst Evol Microbiol 62: 665–670. [CrossRef] [PubMed]
    [Google Scholar]
  35. Zhang L., Zhang Q., Luo X., Tang Y., Dai J., Li Y., Wang Y., Chen G., Fang C..( 2008;). Pontibacter korlensis sp. nov., isolated from the desert of Xinjiang, China. . Int J Syst Evol Microbiol 58: 1210–1214. [CrossRef] [PubMed]
    [Google Scholar]
  36. Zhou Y., Wang X., Liu H., Zhang K. Y., Zhang Y. Q., Lai R., Li W. J..( 2007;). Pontibacter akesuensis sp. nov., isolated from a desert soil in China. . Int J Syst Evol Microbiol 57: 321–325. [CrossRef] [PubMed]
    [Google Scholar]
  37. Zhou Y. X., Xie Z. H., Zhao J. X., Du Z. J., Chen G. J..( 2016;). Pontibacter locisalis Sy30T sp. nov. isolated from soil collected from an abandoned saltern. . Antonie Van Leeuwenhoek 109: 1–6. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001551
Loading
/content/journal/ijsem/10.1099/ijsem.0.001551
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error