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Abstract

A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1 was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0–15 % (w/v) NaCl, at pH 6.0–9.0 and 15–50 °C; optimum growth was observed with 7–8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1 belonged to the genus Lentibacillus , and was most closely related to Lentibacillus garicola SL-MJ1 with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1 was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1 were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1 from the closely related Lentibacillus garicola JCM 30131. Thus, strain WD4L-1 represents a novel species of the genus Lentibacillus , for which the name Lentibacillus populi sp. nov. is proposed. The type strain is WD4L-1 (=CGMCC 1.15454=DSM 101738). An emended description of the genus Lentibacillus is also provided.

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2016-12-01
2019-09-22
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References

  1. Bligh E. G., Dyer W. J..( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37: 911–917. [CrossRef] [PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N..( 2007;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, , 3rd edn., pp. 309–329. Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Collins M. D..( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E.. London:: Academic Press;.
    [Google Scholar]
  4. Collins M., Jones D..( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptido-glycans based on 2,4-diaminobutyric acid. . J Appl Microbiol 48: 459–470.
    [Google Scholar]
  5. Delory G. E., King E. J..( 1945;). A sodium carbonate-bicarbonate buffer for alkaline phosphatases. . Biochem J 39: 245. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Gao J. L., Yuan M., Wang X. M., Qiu T. L., Lv F. Y., Yang M. M., Sun J. G..( 2016;). Paenibacillus radicis sp. nov., a endophytic bacterium isolated from maize root in China. . Int J Syst Evol Microbiol 66: 801–811.[CrossRef]
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R..( (editors)) ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Jacin H., Mishkin A. R..( 1965;). Separation of carbonhydrates on borate-impregnated silica gel G plates. . J Chromatogr 18: 170–173. [CrossRef]
    [Google Scholar]
  10. Jeon C. O., Lim J. M., Lee J. C., Lee G. S., Lee J. M., Xu L. H., Jiang C. L., Kim C. J..( 2005;). Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus. . Int J Syst Evol Microbiol 55: 1339–1343. [CrossRef] [PubMed]
    [Google Scholar]
  11. Jung M. J., Roh S. W., Kim M. S., Bae J. W..( 2010;). Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafood. . Int J Syst Evol Microbiol 60: 1017–1022. [CrossRef] [PubMed]
    [Google Scholar]
  12. Jung W. Y., Lee S. H., Jin H. M., Jeon C. O..( 2015;). Lentibacillus garicola sp. nov., isolated from myeolchi-aekjeot, a Korean fermented anchovy sauce. . Antonie van Leeuwenhoek 107: 1569–1576. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K..( 1988;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  15. Lane D. J..( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackerandt E., Goodfellow M.. Chichester:: Wiley;.
    [Google Scholar]
  16. Lee J. C., Li W. J., Xu L. H., Jiang C. L., Kim C. J..( 2008a;). Lentibacillus salis sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 58: 1838–1843. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lee S. Y., Choi W. Y., Oh T. K., Yoon J. H..( 2008b;). Lentibacillus salinarum sp. nov., isolated from a marine solar saltern in Korea. . Int J Syst Evol Microbiol 58: 45–49. [CrossRef] [PubMed]
    [Google Scholar]
  18. Lim J. M., Jeon C. O., Song S. M., Lee J. C., Ju Y. J., Xu L. H., Jiang C. L., Kim C. J..( 2005;). Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. . Int J Syst Evol Microbiol 55: 1805–1809. [CrossRef] [PubMed]
    [Google Scholar]
  19. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al.( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59: 2114–2121. [CrossRef] [PubMed]
    [Google Scholar]
  20. Marmur J..( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3: 208–218. [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5: 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  22. Miller J. H..( 1972;). Experiments in Molecular Genetics. Cold Spring Harbour, NY:: Cold Spring Harbour Laboratory Press;.
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233–241. [CrossRef]
    [Google Scholar]
  24. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T..( 2005;). Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. . Int J Syst Evol Microbiol 55: 315–320. [CrossRef] [PubMed]
    [Google Scholar]
  25. Pakdeeto A., Tanasupawat S., Thawai C., Moonmangmee S., Kudo T., Itoh T..( 2007;). Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand. . Int J Syst Evol Microbiol 57: 364–369. [CrossRef] [PubMed]
    [Google Scholar]
  26. Rzhetsky A., Nei M..( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. . J Mol Evol 35: 367–375. [CrossRef] [PubMed]
    [Google Scholar]
  27. Rzhetsky A., Nei M..( 1993;). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10: 1073–1095.[PubMed]
    [Google Scholar]
  28. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  29. Sánchez-Porro C., Amoozegar M. A., Fernandez A. B., Babavalian H., Ramezani M., Ventosa A..( 2010;). Lentibacillus persicus sp. nov., a moderately halophilic species isolated from a saline lake. . Int J Syst Evol Microbiol 60: 1407–1412. [CrossRef] [PubMed]
    [Google Scholar]
  30. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K..( 2009;). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. . Int J Syst Evol Microbiol 59: 1137–1142. [CrossRef] [PubMed]
    [Google Scholar]
  31. Stackebrandt E., Goebel B. M..( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bact 44: 846–849. [CrossRef]
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30: 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tanasupawat S., Pakdeeto A., Namwong S., Thawai C., Kudo T., Itoh T..( 2006;). Lentibacillus halophilus sp. nov., from fish sauce in Thailand. . Int J Syst Evol Microbiol 56: 1859–1863. [CrossRef] [PubMed]
    [Google Scholar]
  34. Tanasupawat S., Chamroensaksri N., Kudo T., Itoh T..( 2010;). Identification of moderately halophilic bacteria from Thai fermented fish (pla-ra) and proposal of Virgibacillus siamensis sp. nov. . J Gen Appl Microbiol 56: 369–379. [CrossRef] [PubMed]
    [Google Scholar]
  35. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G..( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25: 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  36. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P..( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60: 249–266. [CrossRef] [PubMed]
    [Google Scholar]
  37. Wang J. L., Ma K. D., Wang Y. W., Wang H. M., Li Y. B., Zhou S., Chen X. R., Kong D. L., Guo X. et al.( 2016;). Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample. . Antonie van Leeuwenhoek 109: 171–178. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wu C., Lu X., Qin M., Wang Y., Ruan J..( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16: 176–178.
    [Google Scholar]
  39. Yoon J. H., Kang K. H., Park Y. H..( 2002;). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. . Int J Syst Evol Microbiol 52: 2043–2048. [CrossRef] [PubMed]
    [Google Scholar]
  40. Yuan S., Ren P., Liu J., Xue Y., Ma Y., Zhou P..( 2007;). Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China. . Int J Syst Evol Microbiol 57: 485–488. [CrossRef] [PubMed]
    [Google Scholar]
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