1887

Abstract

A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1 was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0–15 % (w/v) NaCl, at pH 6.0–9.0 and 15–50 °C; optimum growth was observed with 7–8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1 belonged to the genus , and was most closely related to SL-MJ1 with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1 was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1 were anteiso-C, iso-C and anteiso-C. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1 from the closely related JCM 30131. Thus, strain WD4L-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WD4L-1 (=CGMCC 1.15454=DSM 101738). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001508
2016-12-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/12/5281.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001508&mimeType=html&fmt=ahah

References

  1. Bligh E. G., Dyer W. J.. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol37:911–917 [CrossRef][PubMed]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N.. 2007; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, 3rd edn. pp.309–329 Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Collins M. D.. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp.267–287 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  4. Collins M., Jones D.. 1980; Lipids in the classification and identification of coryneform bacteria containing peptido-glycans based on 2,4-diaminobutyric acid. J Appl Microbiol48:459–470
    [Google Scholar]
  5. Delory G. E., King E. J.. 1945; A sodium carbonate-bicarbonate buffer for alkaline phosphatases. Biochem J39:245 [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  7. Gao J. L., Yuan M., Wang X. M., Qiu T. L., Lv F. Y., Yang M. M., Sun J. G.. 2016; Paenibacillus radicis sp. nov., a endophytic bacterium isolated from maize root in China. Int J Syst Evol Microbiol66:801–811[CrossRef]
    [Google Scholar]
  8. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) 1994; Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Jacin H., Mishkin A. R.. 1965; Separation of carbonhydrates on borate-impregnated silica gel G plates. J Chromatogr18:170–173 [CrossRef]
    [Google Scholar]
  10. Jeon C. O., Lim J. M., Lee J. C., Lee G. S., Lee J. M., Xu L. H., Jiang C. L., Kim C. J.. 2005; Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus. Int J Syst Evol Microbiol55:1339–1343 [CrossRef][PubMed]
    [Google Scholar]
  11. Jung M. J., Roh S. W., Kim M. S., Bae J. W.. 2010; Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafood. Int J Syst Evol Microbiol60:1017–1022 [CrossRef][PubMed]
    [Google Scholar]
  12. Jung W. Y., Lee S. H., Jin H. M., Jeon C. O.. 2015; Lentibacillus garicola sp. nov., isolated from myeolchi-aekjeot, a Korean fermented anchovy sauce. Antonie van Leeuwenhoek107:1569–1576 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Komagata K., Suzuki K.. 1988; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–207[CrossRef]
    [Google Scholar]
  15. Lane D. J.. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp.115–175 Edited by Stackerandt E., Goodfellow M.. Chichester: Wiley;
    [Google Scholar]
  16. Lee J. C., Li W. J., Xu L. H., Jiang C. L., Kim C. J.. 2008a; Lentibacillus salis sp. nov., a moderately halophilic bacterium isolated from a salt lake. Int J Syst Evol Microbiol58:1838–1843 [CrossRef][PubMed]
    [Google Scholar]
  17. Lee S. Y., Choi W. Y., Oh T. K., Yoon J. H.. 2008b; Lentibacillus salinarum sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol58:45–49 [CrossRef][PubMed]
    [Google Scholar]
  18. Lim J. M., Jeon C. O., Song S. M., Lee J. C., Ju Y. J., Xu L. H., Jiang C. L., Kim C. J.. 2005; Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. Int J Syst Evol Microbiol55:1805–1809 [CrossRef][PubMed]
    [Google Scholar]
  19. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol59:2114–2121 [CrossRef][PubMed]
    [Google Scholar]
  20. Marmur J.. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol3:208–218 [CrossRef]
    [Google Scholar]
  21. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  22. Miller J. H.. 1972; Experiments in Molecular Genetics Cold Spring Harbour, NY: Cold Spring Harbour Laboratory Press;
    [Google Scholar]
  23. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  24. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T.. 2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol55:315–320 [CrossRef][PubMed]
    [Google Scholar]
  25. Pakdeeto A., Tanasupawat S., Thawai C., Moonmangmee S., Kudo T., Itoh T.. 2007; Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol57:364–369 [CrossRef][PubMed]
    [Google Scholar]
  26. Rzhetsky A., Nei M.. 1992; Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol35:367–375 [CrossRef][PubMed]
    [Google Scholar]
  27. Rzhetsky A., Nei M.. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol10:1073–1095[PubMed]
    [Google Scholar]
  28. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  29. Sánchez-Porro C., Amoozegar M. A., Fernandez A. B., Babavalian H., Ramezani M., Ventosa A.. 2010; Lentibacillus persicus sp. nov., a moderately halophilic species isolated from a saline lake. Int J Syst Evol Microbiol60:1407–1412 [CrossRef][PubMed]
    [Google Scholar]
  30. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K.. 2009; Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. Int J Syst Evol Microbiol59:1137–1142 [CrossRef][PubMed]
    [Google Scholar]
  31. Stackebrandt E., Goebel B. M.. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bact44:846–849 [CrossRef]
    [Google Scholar]
  32. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  33. Tanasupawat S., Pakdeeto A., Namwong S., Thawai C., Kudo T., Itoh T.. 2006; Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol56:1859–1863 [CrossRef][PubMed]
    [Google Scholar]
  34. Tanasupawat S., Chamroensaksri N., Kudo T., Itoh T.. 2010; Identification of moderately halophilic bacteria from Thai fermented fish (pla-ra) and proposal of Virgibacillus siamensis sp. nov. J Gen Appl Microbiol56:369–379 [CrossRef][PubMed]
    [Google Scholar]
  35. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  36. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol60:249–266 [CrossRef][PubMed]
    [Google Scholar]
  37. Wang J. L., Ma K. D., Wang Y. W., Wang H. M., Li Y. B., Zhou S., Chen X. R., Kong D. L., Guo X. et al. 2016; Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample. Antonie van Leeuwenhoek109:171–178 [CrossRef][PubMed]
    [Google Scholar]
  38. Wu C., Lu X., Qin M., Wang Y., Ruan J.. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16:176–178
    [Google Scholar]
  39. Yoon J. H., Kang K. H., Park Y. H.. 2002; Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol52:2043–2048 [CrossRef][PubMed]
    [Google Scholar]
  40. Yuan S., Ren P., Liu J., Xue Y., Ma Y., Zhou P.. 2007; Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China. Int J Syst Evol Microbiol57:485–488 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001508
Loading
/content/journal/ijsem/10.1099/ijsem.0.001508
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error